XB-CLONE-3181850
Clone Name: IMAGE:7852963
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Gene:
arrdc4
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT393243 [+]
cagaaactctgccccgggggtgaattatccctgcatgtaagcggaaaccgtgcaatgccagaaatgtgtggggctgtcactgtgaccattatatatgaga
atgaagggaagtatggctactgcgccggggagcctgtcactgggtacgtcctgctggaagtgctccgggagatacgggttctgagccttctcctgcgggt
gtgtgggggcgccatgacctgtaagcaggggaaacagctggaagatgcctgggaagctttcccacaggcgacgcactatttgaatgaggagtacagttta
ctaacagagccagcaggtgattgtgaaatactcctgttcccagctggaaatcacaaaattccctttcgttttcaactacctgaaagccctcttgttacaa
gtttttctgggaagtatggtaaagtttactaccaaactacagcggttttgaaaagaccttcagtgcctcctcataccactcacagggagctccgtgtcct
cagtcctatcaacgtcaactctccatctttatatactcctgtagaaagatccaaagaagtaatggtcggttgctggttcttcatgtcaggtccaatatct
gtcaatgccaaaattggaagaaaaggctattgtaatggagaagctattcacatatatgcagatatagaaaatggctcctcccgtttaatagtgcccaaag
ctgccatttaccaaacacagagctttttgctggatgggaagactagaacagtgagacagatgctggccagtgtgcggggaaaccacattgcctctggaag
tgctgactcttggaacggcaaagcactgaagatacccccagtaactccatctnatctggagtgc
3' EST: DT393242 [+]ggaagttaaatctctttatttatgataaaacaagaacccacaggattcatataagttacaaacaccatcttgtttttttgttatcaaaacagaggcttgt
aagagaaatgtgcaaatagaaatgcatagggtttaacaacaagatatcaaagaggtacatttcgtacatttataaaacaaaacatcccatttaatgggga
aatgtaaggccacgatttgatagagattacagcaggcatatgtatgtagatatgtaaaaataataacatggttactatccaacaggtctctttaaagaga
atgcttgctattatataaaagccgatacagtgcatgtttaatattattttacagtagtctaataatgcgtaaattcctcagcctaagttattttggacta
cacgtatgataatcccagcataagtattgcagctactgatgtactggctgtactgtctgcactaggacacaatgttaggctgaagaatgctaattaatgt
ttgtatctcacaaacgatatggaaacaaatacttgtgaaatatatatatatccccccagtttagatgcatgcttatattaatgacagctctcctttaatc
cctgcaggaccacacaatgtatatattagtggaatacatgtctgtatgaaaggcaccctacattttgctttgtatcccaaataaatcagtttcataagat
gcactgatatcaaacgcaagctagctatttcacttttaaccagtttggatttggaataaatattaataaagactcaaagaaggacggaatcgttacagca
ccttaaactatttta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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