XB-CLONE-3181754
Clone Name: IMAGE:7852782
|
Gene:
ckm
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT393306 [+]
ccgaggggggtttacaaaacaaatggtgaaaggatggtaaatgtgcaataatataatctttatcagagaattagatttatcttctcttttatgttgattt
tgaagtttttgttttcttgtgctggatcttcctactacatttctgagattatcccacccacatccattacttatgtattcccctgtagatggccattttt
tgacctctaatgatcttaatggggtctcctgtcccctttgatggtggtaagggttagggtgtttaggctctgtctctagcccctgtcatctacttctgtg
ctggcatcatatcatcaatggactgtcccttctcaagcttcttctccatctcaatcatgagtttcacaccatccactaccatctggacttggtcaacttc
agagaagcccagcctatcagcattggagatatcaaagacacctcctactgctgcagtgtcaacaccacctgtgcctctcttctgcagacgaagcctgccc
agtatctcatcaaatttggggtgtttgctgaggttggggagcttggcatggacacctcctctcaggcctgtgcccaagtttgatgggcaggtaaggacat
aacccagatgttcattccacatgaaggggtgtcctgcttgtttaaagatctcttcaatcttctgaagtccctcacagaagcgtctgaagacttctttcat
gtttcctcctttctgcatggaaatgactctcagatggtcctcttcatttacccacaccaggaaggtcttgttttcattgtgccagataccacgggcatca
ggccagtcacggcccatgccagaggccagaagcagaggggacactggtttgtcaaac
3' EST: DT393305 [+]tagcccgatcttccacatgcctttcggtaacacccataataactggaaattgaattattcctctgaggaggaatatcctgacctcaccaagcacaacaat
catatggccaaggccctgtctgctgacatctacaagaagctcagggacaagcagacaccaagtggcttcaccttggatgatgtcattcagactggagttg
acaacccaggccatcctttcatcatgactgtgggctgcgtagcaggggatgaggaatcctatgaggtgttcaaggacctttttgatcccattattgagga
tcgtcacggtggttacaagccaactgacaaacacaagacagacctcaactttgccaaccttaagggaggtgatgaccttgacccaaactatgtgttgagc
agccgtgtaaggactggccgtagcattaagggatatgccctgcccccccactgcagccgtggggagcgtcgtgccattgaaaaacactccatcaaagctc
tcagcagtctgtctggggaattcaaagggaaatactaccctctcaaggatatgtcagatgcagagcagcagcagctgattgatgaccacttcctgtttga
caaaccagtgtcccctctgcttctggcctctggcatgggccgtgactggcctgatgcccgtggtatctggcacaatgaaaacaagaccttcctggtgtgg
gtaaatgaagaggaccatctgagagtcatttccatgcagaaaggaggaaacatgaaagaagtcttcagacgcttctgtgagggacttcagaagattgaag
agatctttaaacaagcaggacaccccttcatgtggaatgaacatctgggttatgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: