XB-CLONE-3181718
Clone Name: IMAGE:7853024
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Gene:
adsl
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT393330
[+]
cagcgtgtccccgctgactcccgggcccgaagaggttatgaggtataggtcgccgctggtctccaggtacgccagccgggaaatggccttcaatttcagt
gacagcaagaaatttcagacgtggagacggctttggttatggctggggcaggcccaacggagtttggggctacccattacagaagaacagatccaagaga
tggaggccaacctggaaaatattgactttaagatggcagcggaggaagaaaagcgcttgcggcatgatgttatggcacatgtacacacgtttgcacactg
ctgccccaaagctgccccagtcattcatctcggagccacttcttgctacgtgggagacaacactgacctgattgttctacgtgatggttttgacctgctg
cttccaaagcttgctcgagttataagccgcttggctgattttgcagaaagatacgcagagatgccaacgttaggattcacccactaccagccagctcagc
tcacaactgtggggaagcgtgcatgcctttggcttcaggacttgtgcatggacttgcgcaacttggagcgagccaggaatgaccttcgcttccgaggagt
gaaaggtactacaggcacacaggccagcttcctgcagctttttgatggtgatcatgacaaggtggaggaattggacagaatggttacttctaaggcagga
tttaaaagggcttatatagtgactggacaaacttacagccgcaaggtagatgtagaggtgctgtctgtcctggctagtttaggggcgactgttcacaaga
tttgcactgatatccgtttactggcaaacctgaaagagcttgaagagccatttg
3' EST: DT393329
[+]gggaaggcacataatttatttgtacaacagagcagtgaaattcaggactttattttatctgcaaaataaatatttctgattgacacatttcacaggtcca
gctcaatcttcacatccattttactctgataaggactcagcagtggaatcacctcctcctttaaaaacttcagcacctgctgaggtgcccggcctgtgaa
ggacttcggatcaagcagctgttccagatgagcatgaattggggcaaagtaagaatcagattggattctgaagataaggtcattgtctcctccttcttgt
tttaccactgcagccgcttgctgggagagaactcggattcgctcatggcagtcctgcctgtttcctccattcttcaccattgccataatgatattttctg
tggccataaagggcagctcttggcggattcttctttcaatcacttttggatacaccaccaatccttccgaaatgttctgcagagtgctaagaatgatatc
tgcagtgagaaaggcttctgccaaacatactcgtctgttggcactgtcatctagtgtgcgttcaaaccactgaactgaggcagtctgtagcgggttcatg
atgagagtcataaggtggcgagccaagctgcagcagcgttctgagcgcatggggtttctcttgtagggcatggcactagaaccgatctgttctttctcaa
atggctcttcaagctctttcaggtttgccagtaaacggatatcagtgcaaatcttgtgaacagtcgcccctaaactagccaggacagacagcacctctac
atctaccttgcggctgtaagtttgtccagtcactatataagcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: