XB-CLONE-3181663
Clone Name: IMAGE:7852838
|
Gene:
aldoa
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT393366 [+]
cgatggcgtcgccttcaccgaagtcatcaagaaaaaagggggtgtcgtaggcattaaggttgacaaaggtgtcgtccctctggctggaaccaacggtgaa
accacaactcaaggtctcgacggactgtccgagcgctgcgcccagtacaagaaagatggcgctgactttgccaagtggcgctgcgtgctcaagatctcag
aacacactccctctcatctggccatcattgagaatgccaacgtattggctcgctatgccagcatttgccaacagaacggaattgtccccattgtggagcc
cgaggttctccctgatggagaccatgacctgaagaggtgccagtatgtgacagagaaggtcctggctgcagtgtacaaggctctcagcgaccaccacatc
tacctggaagggaccctgctgaaaccaaacatggttacccccgggcatgcctgcaccaagaagtacccccctcaggaggttgctatggcaacagtcaccg
ccctcaggcgcacagtgccccctgctgttggaggaatcaccttcctgtccggaggtcagagtgaggaagaggccaccgtgcacctcaatgccatcaacca
gtgccccctgatcaaaccctggcccctgaccttctcctacggccgtgcccttcaggcctccgccctcaaagcctgggggggcaagaaggaaaacaccaag
aaggcccaggaggagtacgtcaagcgtgccctggccaacagcctggcatgccaaggcaaatacgtagccagcggagaggcgggagctgctgccggcgagt
ctctcttcgtatcanaccacgcctactagacttcctactcctcctagtcactgtggtggccgcttccatttgcctagtgaggggct
3' EST: DT393365 [+]gggtcagaaactgctttattgagggtttgttcagcagtgacacatagaacagtgactcagagccgagaatacagatacaggaggtgcacgtagcccggag
acagacagtcgctctctctcacactcacacacgtcattcacgccctctgctggactgtccaggaatcaccatagcaacgctcagtccgcagtctctctct
ctctccagcaaggggaatggcttagctaatgttccgcaggcgggtctctacattggggaggggctaaccgctctgcaggggaggagcccctcactaggca
aatggaagcggccaccacagtgactaggaggagtaggaagtctagtaggcgtggtttgatacgaagagagactcgccggcagcagctcccgcctctccgc
tggctacgtatttgccttggcatgccaggctgttggccagggcacgcttgacgtactcctcctgggccttcttggtgttttccttcttgcccccccaggc
tttgagggcggaggcctgaagggcacggccgtaggagaaggtcaggggccagggtttgatcagggggcactggttgatggcattgaggtgcacggtggcc
tcttcctcactctgacctccggacaggaaggtgattcctccaacagcagggggcactgtgcgcctgagggcggtgactgttgccatagcaacctcctgag
gggggtacttcttggtgcaggcatgcccggnggtaaccatgtttggtttcagcagggtcccttccaggtagatgtggtggtcgctgagagccttgtacac
tgcagcca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: