XB-CLONE-3181382
Clone Name: IMAGE:7852720
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Gene:
klhl40
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT393553 [+]
ctcttacttcctgcagttcgatcacctggattctgattggctgggaatgccccctgtcccctcagcacggtgcctgtttggtctcggggagtcggacaat
tccatatacctgattggtggaaaggaactaaaagaaggagaacagacgctggacactgttctatgctacgaccgtccgtcatttaaatggggagagtcgg
acccgctaccctatcaagtgtacggtcacaccgtggtgtcccatgataatctggtctacgttcttggaggcaaaggaaatgaaaagaaatgtttaaaaag
agtttgtgtctacaaccccaagaagtttgaatggaaggacctagcaccaatgaaaaccgctcggtcgctattcggaagcactgtccacaagggaaagatc
ttaatcgccgccggagttacagacacgggtttaaccaacaccatcgaagcgtatgatgtcaagaccaataagtgggaggagttcacagagttcccccagg
aacgtagctccctcagcctcgtcagcatgaacgggaccctttatgctattgggggatttgccaccacggagaacgaatcgggggagtttgttccgacaga
actgaacgacatatggaggttcaacgaagaggagaagaaatgggaaggaattcttagagagattcgatatgcgtctggagcgaccttcgttgcagcgcgg
ctcaatatcctgcgactaacgaagatgtaacgacgcgccaggcaatggaagcttaggccaaggatatagagaacatgaacgaactatggggcttactgat
ggtgggacatgttgctgggtcaaaatattcc
3' EST: DT393552 [+]tttgtatctttattttcctttatcatgtaaaaatctcagtagaattagcactgattttgtacgttgagtttgaagattcaaatataaaaatccttgtgca
accataaatatattgtttcctgtacaaacagaggcctatggggataccggtgttgccccgacagccaattaaaagcattagcggctgacctttattgcag
gaagtccgtgctggctgggaattctgggagttttagtatagctatagataaggaaccgaagatccttactttgataggtatcggggaatttaggtacctg
ccctggtccagtaacccatggcaaccaattgctcattttcattgctggaattgtaccagaccagtttactggactgcaggcaattttttgaaaatcattt
agcaactgggtgtgattcagaccagtctaggagagctccgattggggttgtaaggattttttgggggtaaaatgctgcctcaggtgaggaatggttgcat
atgttgagatggggctttggtttcctacaaatagggatgcaccaaatccattattttggggtttgaccgaacaacaaattctttgtgaaagattcatccg
aatactgaactgaacccaaatcggccagacacttggatttggccaaatcctgctgaacctgatcctggattcagtgcatccctaccaatgatataatctg
tgggtatatgtaccataatatcagcttgaactgcctctgtggctaggatggctactgttgggcattgtggccgcacacgtagaaatgacagggagttgat
agctacaaactgtgtaaggaattgtcagctccctggccgaggttgtgcctttctagagcacaggaaaatgttaggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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