XB-CLONE-3181330
Clone Name: IMAGE:7852868
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Gene:
smyd2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT393587 [+]
cgaggatactggccaaacagaaaacccagacagagaggacggcatcggaaaggttcctatcggttaaagattttgaatcccacctcagcaagctcgacaa
cgagaagctcgagttaatccagaacgacattgctgccttgcaccgtttctactccaagaatttgcactattccgacaacgcggcgcaagtgttcctcttt
gcacaggtcaactgcaacggcttcaccattgaagatgaagaactctcccatttgggatccgccatattcccagatgttgcactaatgaatcacagctgct
gccccaatgtgatcgtcacttacaagggaacggtggccgaagtcagagccgtgcaagagatacacgccggagatgaggttttcaccagttacatcgacct
gctctaccccacagaagatcgaaacgacagactgattgactcctatttctttaactgcgactgcagagagtgctctactaaacagaaggatccggcaaaa
ttggaaatccgaaaactgagcgaccctccgagccctcaagttgtgcgagacatgatcaaatacgcacgcaacgtcgtcgaggagttccgaagagcgaagc
actataagactcccagtgagctgctggagatctgcgagctgagtctggagaagatgggctccgtgtttgaggacagcaatgtgtacatgctgcatatgat
gtaccaagccatgggggtctgcctctacatgcaggactgggaaggggccttgaagtatggagagaaaattatcaaaccatacagtaagcactatccccca
tattcccttaacgtggcctccatgtggctgaagctgggccgcctgtatatgggcctggaaaa
3' EST: DT393586 [+]gggttttttgttttttattatcacactgcagtgtaaaacagcaatctcggtgataaaattagcaggcaaactttggttgcattccatttgtaggcaacgg
aaaaacacggcattaaataaaaagtaaaacccgcgtccgggtttcaatcaaaccgtaaaccaagctcagaaatcaattgacgtgaaagttatgggcggct
cctctggaaaccctgtttggcatcccagctccggtcgaggtgaaggaattcagagaaatatacagaacggccctttcattcaggataaaggccaagtgag
cggtggtagaaatattaagagttttacaagcaaacacatgaaaaagaaacccattgggcttcattgcataacgcagaaggacttcaggaccatccctagt
ctgtaggccaccaacttttcttgaacaaagagttcccctaagagtttcagtccaactaaagaggctgaatgcccctgtaggcacagcgttgccatcaggg
aactgaggtcaagccttgtagtccttggactaaaactcccatgattctcagcccatcatgaaagacagaggagcttgcagttgatagtggtgtctcagtc
tcctggatccccattccccgaagcatttatgaagggattgcatagaatctgtgcctcacagctccaattcctttttgatctctgctatataatgatgatc
aggtccatgggctatgtccattatagctatggcctttttcagagcctttgtcccaatagctttcttttccaggcccatatacaggcggcccagcttcagc
cacatggaggccacgttaagggaatatgggggatagtgcttactgtatggt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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