XB-CLONE-3181318
Clone Name: IMAGE:7853060
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Gene:
ccn1
,
stac3
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT393595 [+]
gatccatcgattcgcacttttaatagaacccagttcacaaacaaatgtacaactgacctgctaagaggacagccctgtgtggctatttcatccgtgaaat
aaagaaaccccatggcctacagcattcttagcattttattgggcgcagaccgcaacatagatgcataaaagtagatgtgccaaacctttctccataaact
tcccctctcccccccccacatgaacagaatggcaatggcatgtgcaaaatggaggcagccaacagagtcagcacatatagcagcacactctattccactt
cttcacagtttgatgcatgtgcttatccgacaaaacacaaggaaaggaagcacaaaagctgtgtaattacagcatatattctgtcgtcacaagatgtcac
tttgcacagcctgactctgatataaaacattatgtaaacagttaattgcagcaattcatgatgtctaaatggctctgccttgtataatgctgaatgcatg
agtgacacaaataacaataaacatattttgttatacgggtataggatctgttatctggaaacctgttatccagaaagttctgagttatgtaaaggccatc
ttccatagattccattataagcaaaaaattctaatttttaatttttttaatttttttctctgtaataataaaatggtactttgtacttgatcccaactaa
gctgcatgaatctatattggtgtcaaaaccatccagttgggtttccttaatgttaacattatttttttcagacttaagtcctggtgatctaaattacaga
aagatcccttatctggaaaaccccagatccccaacattctggataatagatattatacctgtaatacaat
3' EST: DT393594 [+]atgtataaataggtaaatagttttattaattgattaattccataaataaattctttaattcattgaatataaaaattaaaccattttacaattattccag
tattacatttgtcacaaaaagctacatttttgataaataaaacacatttaattaaagaaaaaaaaaagacacactgatttctgtttgctaaagaattgct
ggattgtaatagctgccacagatgaacgcttctgaagcgtgctccatcaggaaggaatgtaactttcaacttgttttggaacaagtttacagagtttcgg
tctttcactaaagagaatgtggaaacacttaaccctttgtgtacaagaaaggccaccaacaggctacaatgcaagtgctgaggtcctttctagcattcaa
gggaatggtcaccagaaaaaaaaaatactaagcagataatttactaacggaaaaacagaaaattattattattatgctccaaacaaagaagcaatcagtt
agctccattatgaagcattaaaatgtttcagtctgttgcaacaaagtccagatcacgtatgcacattggaagttcaaagtaccccaaatcgtccatagat
ggcactgcaatagtttgacaacagtaaattccccattttctcaagtttaagcaagtgtccctttaaagcatatagcatttagtctctgaatttgtggata
tcattgaataatctgtagtaaggataggcttcatttgcatgtgggcagttgtagttgcatctacatgactggatcatcatgacattttta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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