XB-CLONE-3181116
Clone Name: IMAGE:7853365
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Gene:
cct8
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT393729 [+]
ccactctccggctcacccacaggtaccatcatggcgctgcacgtccccaaggccccgggctttgcccagatgctgaaggaaggagccaagcactattctg
gattggaagaagcagtgttccggaatatccaggcctgtaaagagctcgctcagactactcgcacagcgtatgggccgaatggaatgaacaagatggttat
caatcatttggagaagctttttgttacaaatgatgctgccaccattatacgggagctagaggtccagcatccggctgccaaaatggttgtcatggcttcc
cacatgcaagagcaggaagttggagacggcactaattttgttctggtctttgctggcgctttacttgagctggcagaagagcttctgcgaatgggactgt
ctgtatccgaggtgatcgaaggctatgagaaggcctgtaagaaagccttggaaatcctccccgacctggtgtgcagctctgccaagaacttacgtgacat
tgatgaagttgcgtcccttcttcagacttccatcatgagcaagcagtatggcaacgagctcttcctttctaagctcattgcccaggcatgtgtctccatc
ctgcccgagtctggccattttaatgttgataacatcagagtgtgcaagattttgggctcaggtatctgtgcctcgtctgtcttgcacggcatggttttca
aaaaggaagcagaaggcaatatttcttctgtgaaagatgccaaaatcgcaatctattcctgtccattcgacggcatgatcactgaaactaagggcaccgt
gctgataaacagcgcgcaagaactgatgaacttc
3' EST: DT393728 [+]atcagaaactgaatgtttattatacagtattatttaacttaagggtaagtgaacaaattgcaaaaaaaaaaaaaaaaaagcgactcggtaatctgtactg
cacacactgattagaccaacggattcagaaaggcggtattcagtcgttttggtcatcgtcccagtccttctttcccgtgggtgcttttgggccgcctgct
ggttttgccatgataatagagccctaagatttgtctggttcatcttgccaatcatccttatcccagtgtccttgctgtttgggggctcttggtccaccag
ctgcctttgccattataatctggtcaaccctaagtacagtgatcgctgcatttgtagccaactttatcccccagtatttccctagatacgtgtccaggat
gttactttccatcatgtctttcactgccgcactctccgcctcaatgtcaaagcccacgttcttgttgccttcctggtgcattgcgtagagtttggagatg
atctcattggccttgacaccagaattttccgccaatgctcttggaatggactcaaatgcttcggcaaactttttaatggcgtactggtcaagcccagggc
aggtctctccataagatgtgatctgttttgccaactcaacttcagtggctccgcctccaggcacaaggcgcttgtcccttgtgaggattttgaaagtatt
cacggcgtcatctactgctcgttccacatcgtccatgagattgtctgtagagccacggatcacaaccgtagcaatggcgccgtcttccttttcgtgcttg
aacacaacaacctgtgtatccncaacttctgaccagtaaacactgtcacagcgccccatttcttctgct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: