XB-CLONE-3181006
Clone Name: IMAGE:7853423
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Gene:
tnxb
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT393802 [+]
ggtttggtaaccttacttcagagttctggctgggaaacattgctctgcatcgtctatcatctttggcaccatatgagttacgtgtagacctccgggcagg
agctgaggctgcctatgctgtgtatgaagattttcgtgttgagggtgaagacaaacactttaggctgcggataggagcatacagaggaaatgcaggggac
tctctgagctatcacaacaatatgattttttcaacacgggaccgtgatgcagagaaaagaatattaccttgttccatttcttatcgtggagcttggtggt
ataagaactgccactatgccaatcttaatgggatgtatggcaacaacaaagaccatcaaggtgtgaactggcacacatggaaaggcttcgaattttcgat
tccattcactgagatgaagatgcggccacagagaacaggcaactggcgccgtctttagagcgctgacagtctgaaaccactaaacttaaaactggaagca
atgtggcatctttggaaaccttacccctatgtcctttctgctaggcccataagatggaacataagtctacaaaactgtatctaattgtaatgtgttttaa
ctccagaactacagatggggaaaatatttcaaaagtgaaaaatctaaaaacactggcctctagaactggagtctagaacccaagtgatggaaacagaaat
tacctagaaatggaaagcagaaaaaacagctatgtgcaggcaatatatatggtctatatatatatacaaaaatgcaatgcagctgaaatctagcagttcc
aggttttagaactctaaaaccataagctataagtattaatataatatagtgtaaatctagaaagaattctggaaaatacaaaaacaatcctcaaagagtc
tacg
3' EST: DT393801 [+]tttttttttttttgaaggtgatatcccatcggtttattagaagcagtttacagaacacaaatcctccatcttttccccctcccatccattccctcttcat
actgtgcttttataaaaatagactgtgtatccgtttgctatatatatatatttatatttatatagtgggtggagggaatggggctctgccacatcctccc
atctccccctccccattttgtggctgtgacacatcttttactccgccccttccccctgagggtgcactcctccccggtagactctttgaggattgttttt
gtattttccagaattctttctagatttacactatattatattaatacttatagcttatggttttagagttctaaaacctggaactgctagatttcagctg
cattgcatttttgtatatatatatagaccatatatattgcctgcacatagctgttttttctgctttccatttctaggtaatttctgtttccatcacttgg
gttctagactccagttctagaggccagtgtttttagatttttcacttttgaaatattttccccatctgtagttctggagttaaaacacattacaattaga
tacagttttgtagacttatgttccatcttatgggcctagcagaaaggacataggggtaaggtttccaaagatgccacattgcttccagttttaagtttag
tggtttcagactgtcagcgctctaaagacggcgccagttgcctgttctctgtggccgcatcttcatctcagtgaatggaatcgaaaattcgaagcctttc
catgtgtgccagttcacaccttgatggtctttgttgttgccatacatcccattaagattggcatagtggcagttcttatac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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