XB-CLONE-3180946
Clone Name: IMAGE:7853285
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Gene:
rpl7a
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT393842 [+]
tgaagaaggccgaggccaagaaggtggtcaaccctctgttcgagaagaggccaaagaacttcggcattggtcaagatatccagcccaagcgtgatctgac
ccgctttgtcaaatggccccgttacatccgactccagcgccagaaggccatcctgtacaagaggctcaaggtcccacctgcaatcaaccagttcacccaa
gccttggaccgtcagacagccacacagctcttcaaactggcccacaagtacagacctgagactaaacaggaaaagaagaaaaggctgctggccagggctg
agcagaaggccgctggcaagggagacgttcccaccaagagaccacctgtactcagagctggtgtcaacactgtcaccacccttgtggaaaacaagaaggc
tcagcttgttgtgatcgcccatgatgtcgatcccatcgagctcgtcgtgttcctccctgctttgtgccgcaagatgggagtcccatactgcattgtcaag
ggcaaggccagactgggccgcctcgtgcacaggaagacctgcaccagcgtagcactcacacaggttaaccctgaggacaaaggtgccctcgccaagttgg
tggaagcagttaagaccaactacaatgaccgattcgatgagatccgtcgtcactggggtggtggtatcctgggccccaagtctgttgcccgcatcgccaa
actggagaaggcaaaagcaaaggagctggccaccaagcttggttaaatgtaccgatcttgttgttttgttctgtacaaa
3' EST: DT393841 [+]gtacagaacaaaacaacaagatcggtacatttaaccaagcttggtggccagctcctttgcttttgccttctccagtttggcgatgcgggcaacagacttg
gggcccaggataccaccaccccagtgacgacggatctcatcgaatcggtcattgtagttggtcttaactgcttccaccaacttggcgagggcacctttgt
cctcagggttaacctgtgtgagtgctacgctggtgcaggtcttcctgtgcacgaggcggcccagtctggccttgcccttgacaatgcagtatgggactcc
catcttgcggcacaaagcagggaggaacacgacgagctcgatgggatcgacatcatgggcgatcacaacaagctgagccttcttgttttccacaagggtg
gtgacagtgttgacaccagctctgagtacaggtggtctcttggtgggaacgtctcccttgccagcggccttctgctcagccctggccagcagccttttct
tcttttcctgtttagtctcaggtctgtacttgtgggccagtttgaagagctgtgtggctgtctgacggtccaaggcttgggtgaactggttgattgcagg
tgggaccttgagcctcttgtacaggatggccttctggcgctggagtcggatgtaacggggccatttgacaaagcgggtcagatcacgcttgggctggata
tcttgaccaatgccgaagttctttggcctcttctcgaacagagggttgaccaccttcttggcctcggccttcttca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: