XB-CLONE-3180883
Clone Name: IMAGE:7853195
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Gene:
cyc1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT393883 [+]
gcctggtgttgcggaggtcgcttggtggggcagtgctgcaggggggacgggcggcctggccggtggctcctcaggccaacatgtccttctccactctgtc
ccggggcaggaaggtggctctctccacattaggtattttggtcgccggaggctccggtttggcgtttgcactccaccaatcagtgaaagccagtgaatta
gagctccacccaccctcctacccatggagccacaacggactcctctcctcgttggatcacgccagcatccggcgcggctaccaggtgtacaaacaggtgt
gcgccgcctgtcacagtatggaatacttggcctttaggaacctcatcggcgtctcccacacggaagcagaggccaaagcgctggcggaagagtttgagat
tctggacggcccggatgagaatggggagatgttcacccggcccgggaagctgtcggattatttccccaagccgtaccccaatgacgaggcggccagagcg
tccaacaatggggcgctgccccccgacctcagctacattgtcaatgccaggcacggcggggaggattacgttttctctctcctgactggttactgcgacc
cccccgctggcgtcagtttaagagaaggactgtattacaacccgtacttccccggccaggccgtcgggatggcgccccctatttataacgaggtcctgga
atttgatgatgggaccccggccaccatgtcgcaggttgctaaggacgtctgcacattcctgcgttgggcttcagaaccggaacacgaccagcgcaagcga
atgggccttaaagtgctgatgatctcttccatcctgatcccactcatctactacatgaagagacaccgatggagcgtgctgaagtcca
3' EST: DT393882 [+]ggtttaaaagatatatttttgtttaatttaacgagcccataatgggatgtaatggacacaatcgtttcttcgcacgagtccctttttcctcctcggtttg
gttctttccggacaaaaacagtgaaaacgcagagaagcaggaacaaaaaattcccccgagacaaaaaataaacataaaaaaaacaaaaagatacggacgc
agcgcctgtgagttacggagccgtgtccggatctacttcctgctcggaggagcaaagggaccgttgcctggcaaatcatccatggcatctgctgaggcca
acgcgcagggggaggggcttcccggtgctgagctgtcggctgccgttacttggggggccggtaggcaatcttcctggacttcagcacgctccatcggtgt
ctcttcatgtagtagatgagtgggatcaggatggaagagatcatcagcactttaaggcccattcgcttgcgctggtcgtgttccggttctgaagcccaac
gcaggaatgtgcagacgtccttagcaacctgcgacatggtggccggggtcccatcatcaaattccaggacctcgttataaatagggggcgccatcccgac
ggcctggccggggaagtacgggttgtaatacagtccttctcttaaactgacgccagcgggggggtcgcagtaaccagtcaggagagagaaaacgtaatcc
tccccgccgtgcctggcattgacaatgtagctgaggtcggggggcagcgccccattgttggacgctctggccgcctcgtcattggggtacggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: