XB-CLONE-3180845
Clone Name: IMAGE:7853437
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Gene:
slc25a30
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT393908 [+]
gtcacctaattcttcggtggcactccttcgtcacttctttgttgcttgagccttctgtttgaaactgctcctcttctcaaaggcaaaatagagacgtcta
gggggaatatgactgctcttaactggaagccctttatatatggggggctagcctctatcactgctgaatgtggtacattccccatagacttgaccaaaac
tcgtctccaggtacagggtcaagctaatgatgccaaatataaggaaatccgatataggggaatgctgcatgcaattgtgaggatatggaaggaagaaggc
gtgaaagcactttattctggaatagcacctgcaatgttacggcaggcgtcgtatggcaccattaaaattggcacttaccagagcttgaaaagattgtttg
ttgattgcccagaagatgaaacgttagtcatcaatgtattctgtggtgtactgtcaggggtggtttcttcctgcattgccaatcctacagatgttttaaa
gataagaatgcaggctcaaggcagtttaatccaaggaggaatgattggaaacttcatcaatatttaccagcaggaagggaccagaggactgtggaagggg
gtatctctcactgcccagagagcagctattgtggttggtgtggaattacctgtatatgatatcaccaagaagcatctgatactttcaggacttatgggag
acacagtctacactcactttttagccagctttacttgcggattggctggagctctggcatcaaatcctgttgatgttgtgagaactagaatgatgaacca
aagggtcatacgcaatgtgtcaaattcaagtacaagggcactctggattgtttattgcagacttggaagaatggagga
3' EST: DT393907 [+]cacagttttaacgtaaatcttgcttgattcattttactcccaaatacacaaattccagagacttacatatttttggccaactgttgggtaaaaatgatat
ttctatggataaatacagaccattaaaggttttcttgtcactctgtaggtggattcccattacgagttccgcttcgtaacatcttgagggtgtaatctat
ggatgcaacttttccaaatcaaagtgacattcctaaaaacactgtatcagtatcatggggcgccctgacatgttacactcaggctttcggcaacaggtac
attacagtactgtaatgaacatttcaagtcatagtggagaagtccagtagttgaacaaagtaggattctgaactttcaaaattgctcagctagaaccaaa
tctcccgtcatcacaggttcagtttcttcagctgttcatatgttatgaagaaaatgatgttccaaggacccagtctcaaccagttaggccaaaatccctt
atacaacgcaaaaaatccttcattcttccaagtctgcaataaacaatccagagtgcccttgtaacttgaatttgacacattgcgtattgacctttggttc
atcattctagttctcacaacatcaacaggatttgatgccagagctccagccaatccgcaagtaaagctggctaaaaagtgagtgtagactgtgtctccca
taagtcctgaaagtatcagatgcttcttggtgatatcatatacaggtaattc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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