XB-CLONE-3180804
Clone Name: IMAGE:7853345
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Gene:
mustn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT393935 [+]
agaagaagaggcctgtgatgaaggaggaagaccttaaaggagcaaaacacaagctggcccaccaggtcccaatcaaatccaaaacataccaggttatgca
ggagtgcgaacaatctggaacaacagccccttctgtgttcagccagaacaggacgggcggagaaacagcctttgacaagccaaaggaaggcccagcgaaa
agtgtctttggttaatggcaaaagaacattcaaataactcggctgcccagcaagtcatttttgttcccaccttctactcattgtgctgcgcacatcatgc
agagtcttacactatatccgcgccagtcatttcactgtctgttatctgtttctaagttactgtaagactttatttcattgctcctatggtcgggctgtgc
tgcattatctgatatactttcagtagagacccatttatatgttttctaaactggctcttcagttattaagtcaacaaaggagccaaatatgattttttag
atttcctatatataaaatggaggaagtgaatacagaatttgtattttctaaaatccaaacccttacaggtccttaggctttggctggaccaaatcagaac
acctcttttcgtcatgtgactttactgggctggggacctaaacacagtcatatcacctactccccatcatcagggtgatatggttcagccctggcatttt
tcctatgtcaacatattaaggtggattcaggactagaccatttcctgcctggtgagggatcacctgctttaattgttgcattaaatgtaaaattaggact
tttcccgttaggtactcatccctaacaaactaagggctgataatctgagcaacctttataggctccaactgt
3' EST: DT393934 [+]acagactttgttcacaagtttaattgtaaaaccaggaaagtttaaaaaaaacaaaccatataagaacatagcgacacaagaatgatcagggctaatacac
aacacacatttacttctcagtgatgctgtgactgggtgctctgttctacaaggtttcatttaatacaccctgacactatctagcaccccgagcagaagcc
tagtgtgatgccagtggcactgagttactacaaggactaagcctgaatgtgtggaagggtgaataggttggggccttatacactgaccaatcatagaact
aactggaacacagtaaatctctcttgccaagtcgagctgtcgctgctggtcaatagaccaatcataactaattgctcctacaaggtcattcacttattga
atagaaggctacaaggtcaactttctctttattctgtcttctaatatcattactggctacttgagatatggacgccgtttttattctacagttggagcct
ataaaggttgctcagattatcagcccttagtttgttagggatgagtacctaacgggaaaagtcctaattttacatttaatgcaacaattaaagcaggtga
tccctcaccaggcaggaaatggtctagtcctgaatccaccttaatatgttgacataggaaaaatgccagggctgaaccatatcaccctgatgatggggag
taggtgatatgactgtgtttaggtccccagcccagtaaagtcacatgacgaanagaggtgttctgatttggtccagccaaagcctaaggaccctgtaggg
tttggattttagaaaatacaaattctgtatt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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