XB-CLONE-3180665
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Clone Name: IMAGE:7853406
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Gene:
aldoa
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT394027 
[+]
cacccgctctcagatagtagcaccatgcctcaccagtacccagcactgacccccgagcagaagaaggagctccatgacatcgccaaacgcattgtggcca
ctggcaagggcatcctggcagccgatgagtccacaggcagcatcgctaagcgcttgagttccatcggggcagagaacaccgaggagaaccgccgcttata
ccgccagctgctcttcaccgccgacgacagagtgaacccctgcattgggggcgtcatcaccttccacgagactctctaccacaagagcgacgatggcgtc
gccttcaccgaagtcatcaagaaaaaagggggtgtcgtaggcattaaggttgacaaaggtgtcgtccctctggctggaaccaacggtgaaaccacaactc
aaggtctcgacggactgtccgagcgctgcgcccagtacaagaaagatggcgctgactttgccaagtggcgctgcgtgctcaagatctcagaacacactcc
ctctcatctggccatcattgagaatgccaacgtattggctcgctatgccagcatttgccaacagaacggaattgtccccattgtggagcccgaggttctc
cctgatggagaccatgacctgaagaggtgccagtatgtgacagagaaggtcctggctgcagtgtacaaggctctcagcgaccaccacatctacctggaag
ggaccctgctgaaaccaaacatggttacccccgggcacgcctgcaccaagaagtacccccctcaggaggttgctatggcaacagtcaccgccctcaggcg
cacagtgccccctgctgntggaggaatcaccttcctg
3' EST: DT394026 [+]tagggcgagaggccttgagtgggtcagaaactgctttattgagggtttgttcagcagtgacacatagaacagtgactcagagccgagaatacagatacag
gaggtgcacgtagcccggagacagacagtcgctctctctcacactcacacacgtcattcacgccctctgctggactgtccaggaatcaccatagcaacgc
tcagtccgcagtctctctctccagcaaggggaatggcttagctaatgttccgcaggcgggtctctacattggggaggggctaaccgctctgcaggggagg
agcccctcactaggcaaatggaagcggccaccacagtgactaggaggagtaggaagtctagtaggcgtggtttgatacgaagagagactcgccggcagca
gctcccgcctctccgctggctacgtatttgccttggcatgccaggctgttggccagggcacgcttgacgtactcctcctgggccttcttggtgttttcct
tcttgcccccccaggctttgagggcggaggcctgaagggcacggccgtaggagaaggtcaggggccagggtttgatcagggggcactggttgatggcatt
gaggtgcacggtggcctcttcctcactctgacctccggacaggaaggtgattcctccaacagcagggggcactgtgcgcctgagggcggtgactgttgcc
atagcaacctcctgaggggggtacttcttggtgcaggcgtgcccggnggtaaccatgtttggtttcagcagggtcccttccaggtagatgtggtggtcgc
tgagagccttgtacactgcagccaggaccttctctgtcacatactggcacctcttcaggtcatggtctccatcagggagaacctcgggctccacatgggg
a
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
