XB-CLONE-3180639
Clone Name: IMAGE:7853074
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Gene:
srp72
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT394044 [+]
tttttttttttttatgagccttgctttatttttttcaacttaaatgataaaataaacatgtgaattcacattttctagttttgcacaacttaaggtttca
catggacatatcttttctcctccataacaaacatggtccttaaaaataaaatgagttgggaggagagctgtagtaaatactaaaagaaaacagctttttt
tcaataagaatttaatttaccctttaaaaattgaaaacttgagtgtaccaaacagaaaattgtggtaagacataaattaatgtagaactttttctgcata
cttttttcatcaccaaccactttttcctcctttctttttcttttgctgctgtttcttcttcgtgcctgcagctcctactggtttttgatgcctgggggga
atgacattgctagaagaggaggaagagccacctggtgctaccaacccaggtcgtggagatgtgggaggactacttgccgttttactagcatccagttcag
ctgctgcagcagatgtggctccttgtgtccctttgcctatctgatccttctttttccccttcttctttccacgataataagagcgctcacgcattgggag
ccaccgttctggatctggtgtaacctttggatcataattcttgggcaactttcctttcttttttttcttcttcttctttttaatgtcggtttgcccttgc
tcctttgcttgttctccagcaacttttgcagcttttttcctaataaaggtagctccggtagaattttctagggcatcaacatctactttcagcttcatgg
tgtcagaagatggcaaatgcttgctaagtatttttgctttttctg
3' EST: DT394043 [+]gagaagctgaagaactttgtagacagtccatttcagaagatgctgatttaactgaagaagatatagaggcagaactggcaataattcatggtcagatggc
gtatattatgcagctccaaggcaaaacagatgatgctctacaactctacaatcaaatcattaagttaaagcccactgatgttggtttgatggctgttgtg
gccaacaatatcatcactgttaacaaggaccaaaacgtctttgactcaaagaaaaaggtgaaacttacaaatgcagaaggagtggagcacaaactgtgta
agaaacaacttcaggctattgaatttaacaaggctctgctttcaatgtatacaaaccaggccgaccagtgtcgaaaaatatctgcaagtcttcagtcaca
ggatccagagcatctgctgcctgtgcttatccaagctgctcagctctgccgggagaagcaacatgccaaagctgtagagatgcttcaggaatttgcagac
cagcatcctgataacgcagctcaaatcaagttaactatggcacagcttaaacttgcacaaggaaacgtgacaaaagcttgtatgatcctcaaaagcatta
aggagcttgaacagacacctggcatggtgtctgcacttgtaactatgcatagctatgatgaagacattgacagtgctattgaagtattttctaatgcaat
cagctggtatcaagaaaaccaaccaatttccccactgcacttgtctttagtaagagaggcagctaacttcaaactaaaacatgngcgga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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