XB-CLONE-3180515
Clone Name: IMAGE:7853228
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Gene:
snta1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT394126 [+]
tctttcgggcaaaggatgaaggtacagcccagtcctggtttaatgccatccacaccaatgccagcgccctggcgccccgtgtgcgggaggatgtgaagca
gctattgggcaaagacatcaaacagatgggctggctcactgagcagctcccccaggatggaaagcgcaatctgctggccctactcacagagaaggatctc
ctgctgtacaacagcctcccgcactccagggagggcttcagcaagcctgcttattgtcacccactcattgccaccaggttggtacactctgggccctcaa
agagctctcctctctacgacacagagctggcatttgctttgcgttctggtacccagaaaggggtggagactcatctgttcagcgtggagacgcagaggga
actggcaacctggacacgggtactggtggacggctgccacagtgcagcagaactcataaaggaggtgaccacagcatgtacatggggtgggaatgagtgt
gccctgtctatacacattgatcaaggtttcactctcttcacggaggaacccggccttaacaaggtgatacacttccaccagccattcgagaagctccgta
tgtcgtctgatgatggtgtcagaattctctatctggaattcggcaaccccgaggcagaacttcaactcgacctgcactcctgtccaaaaaccattgtctt
catcattcactccttcctgtcggccaaagtgtcccgactgggcctattcacctagaacgctgaaagtggcggcaatcttggacctcgcggccgagcgcta
accaaaaccaaccctagggggtgctggagagttagtccaaagtggacagctttccaaatgtcacacaaataatatttgtattta
3' EST: DT394125 [+]cagacactttattgtttattgtacacctccaaagggcaagtaaacaccctccgtttctttccaaacaatcaggatcatgaaacgtcctcccatatatata
tatatctatatccacacgctaaatactttgtaaaataataggttcaagtccagaataaacccccccaaatgcccttattctaatgcagcttaaaaagttg
tgccgggtagcttaatttcaataattaataaaactagatacatatctgcctctatgatcatttatgaatatatattaatatatttatacaaacgccctaa
cctgtgctccgaaggggaaacaattagaccagattgatagctgattgtagagcgggttgtgctgctttataacaacatggcgtggagcagatattgggca
tttaaaggcacagtacttttgtttttccccaaaaacccactgtatatcagagagactgacctggattctccctgaagccatggcattaaacctttgaaaa
agaaatatatatatatataaggcactataattcatggttagggaatcggcagtttacaaagaataaagacgagaggcttcacatttccttgccattaacc
gtatccagacatagtgacatagaagtggttaattctggccatttatggtgtgtaaatacaaatattatttgtgtgacattttggaaagctgtccactttg
gactaactctccagcaccccctagggttggttttggttagcgctcggccgcgaggtccaagattgccgccactttcagcgttctaggtgaataggcccag
tcgggacactttggccgacaggaaggagtgaatgatgaagacaatggtttttggacaggagtgcaggtcga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: