XB-CLONE-3180350
Clone Name: IMAGE:7853386
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Gene:
srp72
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT394235 [+]
cggagcttcggctcctctggcaactttgtggggtgaagtaaataaagccggtcagaacggggattttaccagggcactgaaatcactaaacaaaatattg
caggtggcaaaggatgatgtcacggctcttcattgcaaggtagtttgtcttatccagaatggaaactttaaagaagccctcaatgtaatccacagcaaca
ttaaagttctaacaagtgatgtcattgcatttgaaaaagcatactgtgagtacaggttaaaccgaattgaaaccgcattaaaaaccatagaaggggcttc
acaaaagtcagagaaattaaaagagcttcatggacaagtattatatagattggaaaggtacagtgattgcttatctgtgtaccgagatataattcgaaat
tctcaggatgattatgatgaagagagaaagacaaacttgtctgctgtgttagcagcaatgagtatctgggaaaaaaagacaccggaggactttggcattc
aggagtccacatatgaactgtgctataattctgcctgcacattgataggccagggccacctgcgcaaaggaatgacaaaactgcgagaagctgaagaact
ttgtagacagtccatttcagaagatgctgatttaactgaagaagatatagaggcagaactggcaataattcatggtcagatggcgtatattatgcagctc
caaggcaaaacagatgatgctctacaactctacaatcaaatcattaagttaaagcccactgatgttggtttgatggctgttgtggccaacaatatcatca
ctgttaacaaggaccaaaacgtctttgactcanagaaaaaggtgaaacttacaaatgcagaaggagtggagcacaaactgtgtaagaaacaa
3' EST: DT394234 [+]cttcttcttcttttaatgtcggtttgcccttgctcctttgcttgttctccagcaacttttgcagcttttttcctaataaaggtagctccggtagaatttt
ctagggcatcaacatctactttcagcttcatggtgtcagaagatggcaaatgcttgctaagtatttttgctttttctgcatccaccagtgagtaagcgga
gataagttgggccaaggtatgtatgtctttggggttttgtttccataactgttccagatcactgattgcatcttttttccgcccatgttttagtttgaag
ttagctgcctctcttactaaagacaagtgcagtggggaaattggttggttttcttgataccagctgattgcattagaaaatacttcaatagcactgtcaa
tgtcttcatcatagctatgcatagttacaagtgcagacaccatgccaggtgtctgttcaagctccttaatgcttttgaggatcatacaagcttttgtcac
gtttccttgtgcaagtttaagctgtgccatagttaacttgatttgagctgcgttatcaggatgctggtctgcaaattcctgaagcatctctacagctttg
gcatgttgcttctcccggcagagctgagcagcttggataagcacaggcagcagatgctctggatcctgtgactgaagacttgcagatatttttcgacact
ggtcggcctggtttgtatacattgaaagcagagccttgttaaattcaatagcctgaagttgtttcttacacagtttgtgctccactccttctgcatttgt
aagtttc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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