XB-CLONE-3180160
Clone Name: IMAGE:7853643
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Gene:
st3gal3
,
tob2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT394360 [+]
ttctgtcccaagacaggcttgcatgccttcagatgtccaacttccaagtgaaagtttggttgttcaattaagccagtggccataagaaaagaaagcagta
cagaagtatctgacaactttaagggacaactgtttttgtagtaactacaactttaatccggaaacagacattttgttttaaatggacaagttactgtgcc
aaatgtacctctagtttgggatttgaggtgcaaaaggtacaccacatgcattttgaatgtataaagtattattttagataaaagcatggggttattctgc
gtgggagacagagatatggatggaaggacatcatcacactaatattttcatattcatgcagattacaacatatctctggctgttttatgcagaatcctat
gtaaaactaggacccatgaccgtccagggttaggggcattgttggtgctgctattacttagatgagcaaagaattgttcagtctgtttgttattgaagaa
tgttttaaaatgtatgattaagggtgggaagggttatctggaagattgtaaacaccagaatcttcgatcagtgttaatatttcaccgtggatcagtgttt
gaaatcttaagaacaggtagtttgtgacctgttgtcccatttttgcctttgcatgaataatgtgaagggcgcagactttctgcacacagaaatcatgttc
aatttatcatctacaaaggtgtaagtaattcagacagaaacatcagtaatacatgtcatatcaagaaagaaggcgttatccagaaccagattccaccacc
tttgtgccagacacatgagcaaacagagtggaatcttccatttacatatcagatcatccaaactaataggatttatcattaaattccctgatgtgttgca
gagactgcag
3' EST: DT394359 [+]gaatttttttttttttaagatatataatttgattattgatccttgcaaggcttattcaaaaatcatttcttagaaaaaaataaattaaaaatggatttac
aaactttaaaaaatatagttattatataaaacaattgttaatatattatcttctctgatgccatcactttggccctgatgggcctttttaacataaatct
aatgtttcacataaatacgcctttgctgtggcaggaatagagatggggaaagcagtctcacaatgttcacatcatcattccagtaatgttgtcaatttga
gaaaagatgggctttttaatactgccatgcagaacaatggatatttccgcttttaaacccaacttaaaaagtgctggtaattgtgacttctaattttttc
aatgtgttactgaaaacagtcaaggtattagacaagagtgtttctgtatttaaaaaaaataatatatatatatattaatatatacagcaaccagctgaat
atctgtaagactaacttgtcctttgcatgcataccttcatacagtctaacctgcatgctacaaatctgtgccactctgaagagcacattaggggttcaga
catttgaattagtatatacacaacacttttaagatagtgctcttgtatgttagcctttctataaaaaatatactgtgaaatgtaaattttgtaatatacg
agatcaaacgttcctttgctttgttaaatatctggcttccaatgcattacacaactgaatgctgacttttgacttgaattgttttgggcaaaaataaata
aaaatataaaactgttgtaaacactaaaaaaaaaaaaaaaactatatccccatttgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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