XB-CLONE-3179987
Clone Name: IMAGE:7853469
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Gene:
ckm
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT394474 [+]
tttattgtctccaggcaatcatgggggggtttacaaaacaaatggtgaaaggatggtaaatgtgcaataatataatctttatcagagaattagatttatc
ttctcttttatgttgattttgaagtttttgttttcttgtgctggatcttcctactacatttctgagattatcccacccacatccattacttatgtattcc
cctgtagatggccattttttgacctctaatgatcttaatggggtctcctgtcccctttgatggtggtaagggttagggtgtttaggctctgtctctagcc
cctgtcatctacttctgtgctggcatcatatcatcaatggactgtcccttctcaagcttcttctccatctcaatcatgagtttcacaccatccactacca
tctggacttggtcaacttcagagaagcccagcctatcagcattggagatatcaaagacacctcctactgctgcagtgtcaacaccacctgtgcctctctt
ctgcagacgaagcctgcccagtatctcatcaaatttggggtgtttgctgaggttggggagcttggcatggacacctcctctcaggcctgtgcccaagttt
gatgggcaggtaaggacataacccagatgttcattccacatgaaggggtgtcctgcttgtttaaagatctcttcaatcttctgaagtccctcacagaagc
gtctgaagacttctttcatgtttcctcctttctgcatggaaatgactctcagatggtcctcttcatttacccacaccaggaaggtcttgttttcattgtg
ccagataccacgggcatcaggccagtcacggcccatgccagaggccagaagcagaggggacactggtttgtcaaacaggaagtggtcatcaatcagctgc
tgctgctctgcatctga
3' EST: DT394473 [+]ctagcccgatcttccacatgcctttcggtaacacccataataactggaaattgaattattcctctgaggaggaatatcctgacctcaccaagcacaacaa
tcatatggccaaggccctgtctgctgacatctacaagaagctcagggacaagcagacaccaagtggcttcaccttggatgatgtcattcagactggagtt
gacaacccaggccatcctttcatcatgactgtgggctgcgtagcaggggatgaggaatcctatgaggtgttcaaggacctttttgatcccattattgagg
atcgtcacggtggttacaagccaactgacaaacacaagacagacctcaactttgccaaccttaagggaggtgatgaccttgacccaaactatgtgttgag
cagccgtgtaaggactggccgtagcattaagggatatgccctgcccccccactgcagccgtggggagcgtcgtgccattgaaaaacactccatcaaagct
ctcagcagtctgtctggggaattcaaagggaaatactaccctctcaaggatatgtcagatgcagagcagcagcagctgattgatgaccacttcctgtttg
acaaaccagtgtcccctctgcttctggcctctggcatgggccgtgactggcctgatgcccgtggtatctggcacaatgaaaacaagaccttcctggtgtg
ggtaaatgaagaggaccatctgagagtcatttccatgcagaaaggaggaaacatgaaagaagtcttcagacgcttctgtgagggacttcagaagattgaa
gagatctttaaacaagcaggacaccccttcatgtggaatgaacatctgggttatgtccttacctgcccatcaaacttgggcacaggcctg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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