XB-CLONE-3179939
Clone Name: IMAGE:7853473
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Gene:
ndufv2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT394506 [+]
aggctccggaatggtctcttgagctcggtcagacgatacttgcacacaactactgcatacaatggagcaggcggagcttcatttgtgcacagagatacac
ctgaaaacaatccggacaccccatttgaattcacttcagaaaattacaagagaattgaggctataattggcaactatcctgaaggtcacaaagcaggtgc
tgtaatccctgttcttgacttggcacagagacagcatggatggctacctatatctgctatgaataaggttgctgaagttttagagatgcctgccatgcgt
gtgtatgaggtggctacattctacactatgttcaacagaaaaccagtggggaaatatcacgtccagatttgtacgacaacaccttgcatgctttgcaact
ctgattcaatattggaagctattgaaaagaagctaggaatacatgttggagagacaacatctgataaattatttacactgacggaagttgaatgtctcgg
tgcctgtgtaaatgctccaatggtgcaaataaacgataactattatgaggatctaacaccaaaagatattgaaaatattattgacgagttgaaagctggc
aaagtgccgactccaggcccaagaaatggacgattttcttgtgaaccagcaggaggcctaacatctttgacagggccaccgaaagggcctgggtttggtg
tgcgtgcagacctctaaagtggcactttagatacttacacatgtcanaatatctcanataaaaaattt
3' EST: DT394505 [+]aaattttttatttgagatattttgacatgtgtaagtatctaaagtgccactttagaggtctgcacgcacaccaaacccaggccctttcggtggccctgtc
aaagatgttaggcctcctgctggttcacaagaaaatcgtccatttcttgggcctggagtcggcactttgccagctttcaactcgtcaataatattttcaa
tatcttttggtgttagatcctcataatagttatcgtttatttgcaccattggagcatttacacaggcaccgagacattcaacttccgtcagtgtaaataa
tttatcagatgttgtctctccaacatgtattcctagcttcttttcaatagcttccaatattgaatcagagttgcaaagcatgcaaggtgttgtcgtacaa
atctggacgtgatatttccccactggttttctgttgaacatagtgtagaatgtagccacctcatacacacgcatggcaggcatctctaaaacttcagcaa
ccttattcatagcagatataggtagccatccatgctgtctctgtgccaagtcaagaacagggattacagcacctgctttgtgaccttcaggatagttgcc
aattatagcctcaattctcttgtaattttctgaagtgaattcaaatggggtgtccggattgttttcaggtgtatctctgtgcacaaatgaagctccgcct
gctccattgtatgcagtagttgtgtgcaagtatcgtctgaccgagctcaagagaccattccggagcct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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