XB-CLONE-3179795
Clone Name: IMAGE:7853773
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Gene:
nrap
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT394602 [+]
ttgagaaggtgaaagggaagatgattggccttcgccggctggaggatgatataaatatcgctcattccgtccgtgccgcctccctgctcagtgatgttca
gtaccggaagggctttgagacgactaagacgcagttcaagctgccgatggacatgatacacctgacccatgccaagagagcccagtcgttggccagtggg
caggactataggcacgcgctgcaccattacaccgccctgtccgatgacgtgaggctgctggcggctaagaaggcgcatgaactccagagcgagaaactct
accgctccgacctgagcttcatgagaggggtggggtgcatcacccccggggccctggacattgaggaaagaaagagagccactcagctgatcagtgagcg
taattaccggcagcggccgcagagcctcaaacacaccgccgtggccgactctctggatatccttcatgccaaattcagtaacaaggtaaccaatgagcgc
ctgtacaaggctgtgggggaagatgctctgcaccactacaccgccatcttggatttgcctgagcacctcagggccaagctgaacgcagccaatctcagtg
atataaaatacagggaatcctggctgaatacgagggcccggggccacacactgaccatggaagcgattccattccagtcggccaagtcttccgcagacat
cgccagtgattataaatacaagcacaactatgtgacggagagagggaagcacatcgggtcgcggagcgttctggacgacccccagatgctgcactgcctg
cgcgtggccaagatacagagcgaccagttgtacaggaaagactcccaggacctgcggtcccnataccatc
3' EST: DT394601 [+]ccgaatggagtcgcgtttattccgtacaaacgattctacaaagaagcccaaatctcgtgtataaaaactgatcctgcagtcgctcgagctccgatatacg
tgacaaacaaacacaggggggtgtcggctctgcaaggtcaaactcaccggcgatcccggtacaatggaaacactaccagtcaaggaaatgctattaaact
acactgggggggcaattaaatgtaaagaaaccaaggggggcagtttatatgtggcattaacagcataactgactagttccccactagtaatactattata
tagaacccccacacattctaccacattctgcctcctgcggtggcccaaagtagggcaaagcagggggggcagtttaggacgttccttgtgcacgggggca
aaataacccaggcagtcatgataaataccctgagttacacacaaacatagcaggagctgccccgttcctttctatctgccccggggaattcccaagcgat
cggaccggcaccaaatgttcatttcttttttgccaaaattttcttcctcttcacctgaaggatctcggaggcatcgggattaacatcgctgtgtggggac
tggtaccccgtctcgtctgctgaataaggagacactgacgcccggtctgactctgtgccatctccgtatgacagttgggccgcccggcgagccagttcta
ctttgtagtagccagggggggtccaaccaacccctttcatccagttcagatctgatttgtacttaatatcgctctgcaggctgtgggcttgcttggcgtg
tgtcatggctagctgctgggggt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: