XB-CLONE-3179789
Clone Name: IMAGE:7853487
|
Gene:
spon2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT394606 [+]
aaaacaataaataatgacgaagtgaagggcattcggaagttcttaacaacagcctgaaaagctgcggtgcatcactttattgccctgttttctcaacata
taatatagtccctattctaccacacacaatcactgcacccgataaacctcctgaagttggcactgattctgcacttgagtaccttaacctgctttgttga
gggtatggccaggtgatgttactttaaagcaagctgagggagtcaatgtgtcagaaaaagtgcagagggttttttatgaacaaagtgctggccgcttgca
ctttgaacatacaaaagttttgtctctgtaaaattgtaaatttccattatctgtaaataatgtaaatatgtaggtgttgcctgcaggagggtagttgcaa
gcagcacctaatattcacagtaattcaatccaaatacttggatttcgtatagttcgtgtagagcatcctctcttgggtgtaggtttgtcttcaacaggga
gtttctcaacctgctgaggtttgcttagacatcaacgtagagttgaggcacatagtttaatggcaggcaaggtattcgctccctaggaaactttctcctg
cccaccgttggttccagtttcattttaaaacgtcataaaatatatggtacagaactccataaccggtaaactcggctatatacagtatgtacatgtcagt
taaaaatgtatcaatttccctattcaaataggaatatgtgatagcgtanatcttttcgactttgtcttatacaggtaggtacaaaaatagcttttatatt
tacaaatcagttcatctagttctagtgaatcacttgtaaaattacatgtgaatgtaaacgataaaatgcttaaatgtgtcggacattaatattcacagat
gcagaatcggac
3' EST: DT394605 [+]aggcccaaaattgaagctcttcccaatgatgtcagcattgacgaaggaaaagtacttactgtggcttgtgccttttctggagaacccacaccagatattg
catggtctaaggctggacatccagttcccactcaagaacaaaaaggcagagttcacattgaaacttctgaagacttaaccactcttatcatcatggacat
tcagaagaaagatggcggacattacactcttaacctgtcaaacgacttcgggtccgattctgcatctgtgaatattaatgtccgaacacattaagcattt
tatcgtttacattcacatgtaattttacaagtgattcactagaactagatgaactgatttgtaaatataaaagctatttttgtacctacctgtataagac
aaagtcgaaaagatttacgctatcacatattcctatttgaatagggaaattgatacatttttaactgacatgtacatactgtatatagccgagtttaccg
gttatggagttctgtaccatatattttatgacgttttaaaatgaaactggaaccaacggtgggcaggagaaagtttcctagggagcgaataccttgcctg
ccattaaactatgtgcctcaactctacgttgatgtctaagcaaacctcagcaggttgagaaactccctgttgaagacaaacctacacccaagagaggatg
ctctacacgaactatacgaaatccaagtatttggattgaattactgtgaatattaggtgctgcttgcaactaccctcctgcaggcaacacctacatattt
acattatttacagataatggaaatttacaattttacagagacaaaacttttgtatgttcaaagtgcaagcggccagcactttgttcataaaaaacctctg
cactttttctgacacattgactccctcagcttgctttaaagtaacatcac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: