XB-CLONE-3179604
Clone Name: IMAGE:7853696
|
Gene:
arrdc2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT394728 [+]
aacctttctcctccacatcattctagcatgggctgtttgctagaagggccattctatgcttacatccaagaattcagaaacagacctcctcctctttact
ccgaggttgatcctaatcctcccactgcagacatcagaccacgttgcatgacctgctgaaagatgactgccagcaaactgaattgtctatgtaacgtaaa
gcagttgagcttagactgaaaatgtcctcacctccaactggaggtttcctctgggagaactgatccagaggtaggaggaaacagacttatgcctgtcttc
ccatggagaaatgaagctttccatgcaagagatgggtctggtgttccagtgcatcaccagtgtctcccagaaagcagtctgtaaaaggaagaggaggcga
ggaatgatcattttaaccactattgaacctttgagtcagtgattatagtctgcttataatgtgattataatctgtttattgagctctggggaaaaacagg
ctttctgcacttctaaagaaaaaactaagctgttatgagatgttactaaaactgtaatagtacagcatttttatttttttctagacaattgtacattcta
gctgaaattgtcaaatgcctgacatcacccagcatgttgcaggctttgcttgcccagagcacagaaaccaaactgttaagggctaagcatttcacacaac
caaagttaatgtttacagtacagtcagtctggttcatagtttgcttagcaggtgctgagctaaagaatgttctgcctttttcattctttaaacattgtag
agcagatattttccccctgtaatttctacagtgcctgcatacaaccaaagattgaactgggatctaaaaaagaaaaagtttac
3' EST: DT394727 [+]gttttacagggaatacagggaatatctggaaatttcccactgaagtattttttcaacagcccgagggtcatgagtaatcccatctcagagtttaaagtcc
tgacttatatgtaaggtcacttaatagaatgaaaaggcactgtagcaccaaattccgctttcaaagcaatcctctttcattaatactgaaaaaatataag
gtgattttacagattgaataaataataaatcaatgtgcttgaaatataaacttacaaagcttacactattacaattttataaagttcactttcaactcat
aattaatatgtatcttttttttttctcccttaagaggacttaaaaaaaaaaaaaaaaaaggtttgctttaaggctactgctgccatggcatggaataaca
aattaaggcagatactgtgtgtatagctattccataggtcaagcagatgtcataataaaagtaaacttttttcttttttagatcccagttcaatctttgg
ttgtatgcaggcactgtagaaattacagggggaaaatatctgctctacaatgtttaaagaatgaaaaaggcagaacattctttagctcagcacctgctaa
gcaaactatgaaccagactgactgtactgtaaacattaactttggttgtgtgaaatgcttagcccttaacagtttggtttctgtgctctgggcaagcaaa
gcctgcaacatgctgggtgatgtcaggcatttgacaatttcagctagaatgtacaattgtctagaanaaaataaaaatgctgtactattacagttttagt
aacatctcataacagcttagtt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: