XB-CLONE-3179548
Clone Name: IMAGE:7853558
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Gene:
cd44
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT394765 [+]
caaatctactttaggtggcccaagcttgggggggtccagcataggatcatcttctgaaatttcaggcaacctttatactgagttaggttcctaacaatac
atggtatatttaattcaatcaaactgtttcagaagaacggtagaatgaggggcccaggctgaaggctagggtgctcttcagcctgaagatcagtcagggt
tagatattggttgaagaacagcaggatgaatgatagttgacattgaggcccctattaaaaatacctgcttatttttgtacagattctatttaagtttcct
taccgtatctcgtatccttaccgtatcacaccctgggtgtcagtttcaatgaacaagagaagaagcattggatctcaaaatatctcaagccttcacatga
gtggctctgattggccggcataggccatcgtcaatttaggagcttcagtggactctcgtactgtgtatatgctgggaatactttagtgctgaaagtttca
gacgaatgtatattattaacccttcactcccaaagagttctcattgaacagcgcctctggtaggaaaggggggtggagatgatgaggagaagcttattgt
atatagaggttccaagggcttaacagtttcatgtatggaagatattttatttagtgtgtgtctttttgtaggagctgtgtgaaagggtggctgggttttc
aaggggtaactagccctttatttcatggtgaagggggttgaacccaatctgttagggtactagcgcaattttgtatatttgggcccctgaacaacagccc
aggctaccggattactggtttaactgggggcacatt
3' EST: DT394764 [+]cgagaggctttttttagtgcagaacatgtgcttttattattggggtatttcctcatgtgttctacagatatcccgacccacacagaatcatggttccgct
cgcgggctgtatctgggctctttacaagggataaagaattgttcttccgtaatttgaatactcatttacatagtcccgcccaccagtgtaacaggggtca
ttggaagaaataagtgctccaaagggtaagtcaaaccatcgcacggcacaaagttgggtgtttctgtgcctataatgttccatatcaccactatccagca
ggtcttaatggatcccatttgtaagactgatgtgtctccccaatctgagagagcaaccaatcacattggacctttgttaatatcagaagatcattcttac
catttacatggtgcgactttacccctagggcaatatataaaataaaaagtaggtggggtcatgtttttgggctcctaaaaattttttgtcactttattta
ttacattgcccaatgtgcccccagttaaaccagtaatccggtagcctgggctgttgttcaggggcccaaatatacaaaattgcgctagtaccctaacaga
ttgggttcaacccccttcaccatgaaataaagggctagttaccccttgaaaacccagccaccctttcacacagctcctacaaaaagacacacactaaata
aaatatcttccatacatgaaactgttaagcccttggaacctctatatacaataagcttctcctcatcatctccacccccctttcctaccagaggcgctgt
tcaatgagaactctttgggagtgaagggttaataatatacattcgtctgaaactttcagcactaaagtattcccagcatatacacagtacgagagtccac
tga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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