XB-CLONE-3179530
Clone Name: IMAGE:7853464
|
Gene:
mustn1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT394777 [+]
gtgccccagttaagaagaagaggcctgtgatgaaggaggaagaccttaaaggagcaaaacacaagctggcccaccaggtcccaatcaaatccaaaacata
ccaggttatgcaggagtgtgaacaatctggaacaacagccccttctgtgttcagccagaacaggacaggcggagaaacagcctttgacaagccaaaggaa
ggcccagcgaaaagtgtctttggttaatggcaaaagaacattcaaataactcggctgcccagcaagtcatttttgttcccaccttctactcattgtgctg
cgcacatcatgcagagtcttacactatatccgcgccagtcatttcactgtctgttatctgtttctaagttactgtaagactttatttcattgctcctatg
gtcgggctgtgctgcattatctgatatactttcagtagagacccatttatatgttttctaaactggctcttcagttattaagtcaacaaaggagccaaat
atgattttttagatttcctatatataaaatggaggaagtgaatacagaatttgtattttctaaaatccaaacccttacaggtccttaggctttggctgga
ccaaatcagaacacctcttttcgtcatgtgactttactgggctggggacctaaacacagtcatatcacctactccccatcatcagggtgatatggttcag
tcctggcatttttcctatgtcaacatattaaggtggattcaggactagaccatttcctgcctggtgagggatcacctgctttaattgttgcattanatgt
aaaattaggacttttcccgttaggtactcatccctaacaaactaagggctgataatctgagcaacctttataggctccaactgta
3' EST: DT394776 [+]gactttgttcacaagtttaattgtaaaaccaggaaagtttaaaaaaaaaaaaccatataagaacatagcgacacaagaatgatcagggctaatacacaac
acacatttacttctcagtgatgctgtgactgggtgcactgttctacaaggtttcatttaatacaccctgacactatctagcaccccgagcagaagcctag
tgtgatgccagtggcactgagttactacaaggactaagcctgaatgtgtggaagggtgaataggttggggccttatacactgaccaatcatagaactaac
tggaacacagtaaatctctcttgccaagtcgagctgtcgctgctggtcaatagaccaatcataactaattgctcctacaaggtcattcacttattgaata
gaaggctacaaggtcaactttctctttattctgtcttctaatatcattactggctacttgagatatggacgccgtttttattctacagttggagcctata
aaggttgctcagattatcagcccttagtttgttagggatgagtacctaacgggaaaagtcctaattttacatttaatgcaacaattaaagcaggtgatcc
ctcaccaggcaggaaatggtctagtcctgaatccaccttaatatgttgacataggaaaaatgccaggactgaaccatatcaccctgatgatggggagtag
gtgatatgactgtgtttaggtccccagcccagtaaagtcacatgacgaaaagaggtgttctgatttggtccagccaaagcctaaggacctgtaagggttt
ggattttagaaaatacaaattctgtattcacttc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: