XB-CLONE-3179258
Clone Name: IMAGE:7853822
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Gene:
spon2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT394957 [+]
tgaagctcttcccaatgatgtcagcattgacgaaggaaaagtactaactgtggcttgtgccttttctggagaacccacaccagatattgcatggtctaag
gctggacatccagttcccactcaagaacaaaaaggcagagttcacattgaaacttctgaagacttaaccactcttatcatcatggacattcagaagaaag
atggcggacattacactcttaacctgtcaaacgactttgggtccgattctgcatctgtgaatattaatgtccgaacacattaagcattttatcgtttaca
ttcacatgtaattttacaagtgattcactagaactagatgaactgatttgtaaatataaaagctatttttgtacctacctgtataagacaaagtcgaaaa
gatttacgctatcacatattcctatttgaatagggaaattgatacatttttaactgacatgtacatactgtatatagccgagtttaccggttatggagtt
ctgtaccatatattttatgacgttttaaaatgaaactggaaccaacggtgggcaggagaaagtttcctagggagcgaataccttgcctgccattaaacta
tgtgcctcaactctacgttgatgtctaagcaaacctcagcaggttgagaaactccctgttgaagacaaacctacacccaagagaggatgctctacacgaa
ctatacgaaatccaagtatttggattgaattactgtgaatattaggtgctgcttgcaactaccctcctgcaggcaacacctacatatttacattatttac
agataatggaaatttacaattttacagagacaaaacttttgtatgttcaaagtgcaagcggccagcactttgttcataaaaaaccctctgcactttttct
gacacattgactccctcagcttgctttaag
3' EST: DT394956 [+]atggtgcagaaaacatggctttattgcaatcaaagagctatcttcgggaggttcaataaataataatgctacaataacataaaataaaacaataaataat
gacgaagtgaagggcattcggaagttcttaacaacagcctgaaaagctgcggtgcatcactttattgccctgttttctcaacatataatatagtccctat
tctaccacacacaatcactgcacccgataaacctcctgaagttggcactgattctgcacttgagtaccttaacctgctttgttgagggtatggccaggtg
atgttactttaaagcaagctgagggagtcaatgtgtcagaaaaagtgcagagggttttttatgaacaaagtgctggccgcttgcactttgaacatacaaa
agttttgtctctgtaaaattgtaaatttccattatctgtaaataatgtaaatatgtaggtgttgcctgcaggagggtagttgcaagcagcacctaatatt
cacagtaattcaatccaaatacttggatttcgtatagttcgtgtagagcatcctctcttgggtgtaggtttgtcttcaacagggagtttctcaacctgct
gaggtttgcttagacatcaacgtagagttgaggcacatagtttaatggcaggcaaggtattcgctccctaggaaactttctcctgcccaccgttggttcc
agtttcattttaaaacgtcataaaatatatggtacagaactccataaccggtaaactcggctatatacagtatgtacatgtcagttaaaaatgtatcaat
ttccctattcaaataggaatatgtgatagcgtaaatcttttcgactttgtcttatacaggtaggtacaaaaatagcttttatatttacaaaatcagtcat
ctagttctagtgaatcacttgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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