XB-CLONE-3179050
Clone Name: IMAGE:7853937
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Gene:
klhl40
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT395095 [+]
aaaaatctcagtagaattagcactgattttgtacgttgagtttgaagattcaaatataaaaatccttgtgcaaccataaatatattgtttcctgtacaaa
cagaggcctatggggataccggtgttgccccgacagccaattaaaagcattagcggctgacctttattgcaggaagtccgtgctggctgggaattctggg
agttttagtatagctatagataaggaaccgaagatccttactttgataggtatcggggaatttaggtacctgccctggtccagtaacccatggcaaccaa
ttgctcattttcattgctggaattgtaccagaccagtttactggactgcaggcaattttttgaaaatcatttagcaactgggtgtgattcagaccagtct
aggagagctccgattggggttgtaaggattttttgggggtaaaatgctgcctcaggtgaggaatggttgcatatgttgagatggggctttggtttcctac
aaatagggatgcaccaaatccattattttggggtttgaccgaacaacaaattctttgtgaaagattcatccgaatactgaactgaacccaaatcggccag
acacttggatttggccaaatcctgctgaacctgatcctggattcagtgcatccctaccaatgatataatctgtgggtatatgtaccataatatcagcttg
aactgcctctgtggctaggatggctactgttgggcattgtggccgcacacgtagaaatgacagggagttgatagctacaaactgtgtaaaggattgtcag
ctcct
3' EST: DT395094 [+]ttgctgtaactccgacgctgcccgagctctgagggcaaaggagaaccaagccatggccttaccgacccagcaggctgatgagttgcgcctctaccagcaa
actctgctccaagatggactcaaggacatgctggaccacgacaactttatagactgtgttctaaagatccagggcaaggagtttccctgccaccgcctgg
tgctggccgcctgtagcccttacttccgagccatgttcctttccaacctggaggagggcaagaagaaggaaattgacctagaagatgtcgatccagacgt
gatggggaagatcctccattatatctacacttctgagatcgagatcacagagaagaacgtccaggacatattctcggtggccaacatgttccagatcccc
tcgatattcacagtgtgtgtgtctttcctccagaagaagctctgccttagcaactgtctggccatattccgcctgggcttgctgttggattgtccccgtc
tggccgtctccgccagggactttgtatgcgaccgattccacctgattgccagagacgaagagttctacgacctttcccccgatgagctgattgccgttat
ctccagcgactccctcaacattgagaaggaagaagaagtctttgaggtggtcctgaaatgggcgttgaaagagaaagaaaaacgcgccaaggcgttgccc
atcatctttgagagcattcgcttccgccttataccccaagactatataaagaacaaggtggagaagcacgagttggtcaagtccgacaaggaactcttga
agaaactccagatggtcaaagacgccca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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