XB-CLONE-3178852
Clone Name: IMAGE:7853859
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Gene:
tsc22d3
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT395226
[+]
ctacttctaggagtcccataaaggacaggatatacgttttactggagctctatatattgaggagccgccgtgccaaaaacatgagcacagaaatgtgtaa
cttcccaatggaggtggccgtgtatgagctgcacaatttctccatctcgtttttctcctccttactgggggctgatgtggtgtccgtaaaactggataac
agtgcatcgggtgccagtgtcgttgccatcgacaacaagattgagcaagccatggatttggtaaagaaccatcttatgtacgctgtgcgtgaagaggttg
aggtgctgaaggaacagattaaagagctggtggagaagaactcgcagcttgagaaagagaatcatctactaaagaacctggcaagcccagagcaaatgaa
gaagttccagtctcggctccctcaagatgaaactccaatcaagacaaaactagatttccttcgcgcatctcagaaggctggctctgcagtctaagtggct
ctgtctttggagtgggcagagccactgaacttttcctgcgtctatatatgagaaaaaaatgagatttttttcccccctctttaaagtgtcacctcggctt
ggagggtcgttgactagccaaagctggcatgtgccggggctagcgtgggcctcctcacaagtcttttcttcaagagaagagtgagagaagctagacatgg
actctttaagaagagtaccagccagagagagctggctcggcatgaatgttgcctttctttggcgccaggcttgngaagcanagccaggaaacaagcagga
catgctaaagatgaccagagactgcttctg
3' EST: DT395225
[+]atataaaaaaacggtttattgcagctttccactgaattatgtacaatccagaaatctcagacagtaatgttaaacagcagaaaacattattcgtccaaca
agcccttttatggccattgctgggagctgtagtcctgaagtttttaggaatattgcaaaaaaagtccacagtctattaattttcccggattttaaagtgt
cttgtgtgagtctttcaggcggcttttcccaccattgtgctctggcatagagtcagacaggtacatttatattctacacatacataaaaagacaaagtaa
aaaaaaaaaaaaaagtctgtgtagcatattcccaagttttcaaagagaacattttggcaagtgctaaaattacatactagctatgtaaacttaaactaac
aagcccagtagtgggtggggtctaaagagactgccaccgatcactagtgtgggcaactgtgagccaacgtataacaatctccattataaagcactaaagt
gccctatgtattatactttgtactgaactgttactagggtaactgttccttatcctcattccaggaggttaacgctctctgcttcccactatggtacagt
ttataaaaacacacagagagggagatctcctcatcaactttgtattccatgcccagagaactcttgcagaagcagtctctggtcatctttagcatgtcct
gcttgtttcctggctttgcttcccaagcctggcgccaaagaaaggcaacattcatgccgagccagctctctctggctggtactcttcttaaagagtccat
gtctagcttctctcactcttctcttgaagaaaagacttgtgaggaggcccacgct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: