XB-CLONE-3178804
Clone Name: IMAGE:7854103
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Gene:
atg2b
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT395258 [+]
tttacagaataaggactacaatttacatatttctgctgtatccaggctttccagagtcaaatagcaacacagaacaactcattaaacaacattaaaccag
agtgcattgcagaggagaatccaaggagtccatcaagcaaacatccaaacacacttatattactttgtcgtctgccagattggaaagtttgttcggacgc
aaggttcattactagctattaattgctgagggtatgttgcaaatagcacacatgccaaaagaatggaaaccatccagctgaaatgacccccagtttagaa
cctgttcagagaaccatgcatacagatgaacaactgaatgagtggggggcaaagtcacagatgtcggtgtaaataataattttcaaaagctatgtagtgt
ctattttttccctttttgtcatcaaaaaagtaaccagattcctgatccaaatttgctgaaacatcacaaattgtaaccttgagggggagggggtcttcca
ttacttctttgtcaggcaaacgcacagccacaaagcatgaaactaaccctcttccagcctccatttctgagactcttcctctcgaacatcaggcctgatt
tgattcctcataccacccaaaacatttgaagtggcttcagtcgcaactattagaggtttaacaactgcaggaggaatctgtcttaaaactcctccaacag
ctcctgtcacacctctgttttcatgctcacgggctgctgtctcatagatggtctgagccgtatctgtaatgccctctttaacacagtatatgctttagcc
accccttcccttagatccacaggctgatgcaccaatcgatgatgggaaaatcgttttgctctcttaagctcgacagggcttggt
3' EST: DT395257 [+]gcagcttgttcagggactgaaggacttggtgtggctgcctattgaacagtataggaaagatggtcgcatcgtccgaggtttccagaaaggagctgcatct
tttggaacgtctactgccatggcagctctggaactgactaacagaatagttcaaactattcaggctgctgcagaaacagcgtatgatatggtgtcccctg
gaccaagccctgtcgagcttaagagagcaaaacgattttcccatcatcgattggtgcatcagcctgtggatctaagggaaggggtggctaaagcatatac
tgttgttaaagagggcattacagatacggctcagaccatctatgagacagcagcccgtgagcatgaaaacagaggtgtgacaggagctgttggaggagtt
ttaagacagattcctcctgcagttgttaaacctctaatagttgcgactgaagccacttcaaatgttttgggtggtatgaggaatcaaatcaggcctgatg
ttcgagaggaagagtctcagaaatggaggctggaagagggttagtttcatgctttgtggctgtgcgtttgcctgacaaagaagtaatggaagaccccctc
cccctcaaggttacaatttgtgatgtttcagcaaatttggatcaggaatctggttacttttttgatgacaaaaagggaaaaaatagacactacatagctt
ttgaaaattattatttacaccgacatctgtgactttgccccccactcattcagttgttcatctgtatgcatggttctctgaacaggttctaaactggggg
tcatttcagctggatggtttccattcttttggcatgtgtgctatttgcaacatacc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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