XB-CLONE-3178771
Clone Name: IMAGE:7853915
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Gene:
ndufs3
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT395280 [+]
ctcccgcctgaccggcgctaaatccgtgatgttggctggggtgcgggctgcccgggggctcatcaggggagcatgtgctttccgctctcttccacaagtc
cgactggagggcacgaccacagagacaagacctacggtgaggccacaagataaagtgcagttgaaccaactttcagcctttggcgagtatgtagcggaga
tcctccccaagtatgtgcagcaagttcaggtgacctgtaatggagagctggaagtcatggttcacccagatggggttataccagtcctcacgtttcttcg
cgaccacacaaacgcccagttcaaatcactggccgatctgactgctgttgacgtcccagcaaaaacaaaccggtttgaaatagtgtacaaccttttgtca
ttgcggttcaacacccgtatgcgcgtgatgacctacaccgatgagctgactcctgttgagtcggctgtgcctgttttcgaggctgcaaactggtatgaga
gagaggtttgggatatgtacggtgtatttttcgcaaaccatcccgatttgagacggatccttacagactacgggtttgaaggccatcccttccgtaaaga
tttcccactgtctggatatgtggaggtgcgctatgatgatgaggtaaagcgagtggtggcagagccggtgcagctgtcccaggagttccgcaagtttgac
ctgagcagcccatgggaagcctttccggcacatcgtcagcagcctgaaagcccaaaactggagcccggggaaaccaaataagtctcccgncagttgtgct
tctagttcttacaagttatttatgtatataaaataaagacctaaactttgt
3' EST: DT395279 [+]gaaaccacaaagtttaggtctttattttatatacataaataacttgtaagaactagaagcacaacttgcgggagacttatttggtttccccgggctccag
ttttgggctttcaggctgctgacgatgtgccggaaaggcttcccatgggctgctcaggtcaaacttgcggaactcctgggacagctgcaccggctctgcc
accactcgctttacctcatcatcatagcgcacctccacatatccagacagtgggaaatctttacggaagggatggccttcaaacccgtagtctgtaagga
tccgtctcaaatcgggatggtttgcgaaaaatacaccgtacatatcccaaacctctctctcataccagtttgcagcctcgaaaacaggcacagccgactc
aacaggagtcagctcatcggtgtaggtcatcacgcgcatacgggtgttgaaccgcaatgacaaaaggttgtacactatttcaaaccggtttgtttttgct
gggacgtcaacagcagtcagatcggccagtgatttgaactgggcgtttgtgtggtcgcgaagaaacgtgaggactggtataaccccatctgggtgaacca
tgacttccagctctccattacaggtcacctgaacttgctgcacatacttggggaggatctccgctacatactcgccaaaggctgaaagttggttcaactg
cactttatcttgtggcctcaccgtaggtcttgtctctgtggtcgtgccctccagtcggacttgtggaagagagcggaaagcacatgctcccctgatgagc
ccccgggcagcccgcaccccagccaacatcacggatttagcgccggtcaggcggga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: