XB-CLONE-3178700
Clone Name: IMAGE:7853921
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Gene:
ppp3r1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT395327 [+]
gtcagtctacaaccgccctgtgcgcccagcctgagccttgttccgcagcaccgagtggaacagggagagtgccggggattcctgaggccgacacctgagc
tgtgatagtgaagcgagcgccgctcagccgccaacatgggaaatgaagcaagctatccgctggaaatgtgctcacatttcgatgctgatgagatcaaaag
gctagggaaaaggttcaagaaactggatttagacaactctggttccctgagtgtggaggagttcatgtcgttaccagagcttcaacagaaccctcttgtg
cagcgagtaatagacatattcgacacggatggaaacggagaagtagattttaaagagtttatcgaaggagtgtcccagttcagcgtcaaaggtgataagg
agcagaaactgaggtttgcttttcgtatatatgatatggacaaggatggctacatctccaatggtgagcttttccaggtactgaagatgatggtgggaaa
caatctcaaagacactcagttacagcaaatagtagacaaaacgattatcaatgcagataaagatggggatggaaggatatcctttgaagaattctgtgct
gtcgtaggaggcttagatatccacaaaaagatggtggtggatgtgtgactgacctgagcagcacccaacacacttgctttcttctccatctctgaagatc
tgctcaagacgtccagcaatgctctctgtgtattttcaatggaagtatttttctctgtgaagccacattttccaacacgagcctcatgaagccaacttag
tggtattgaactttttttgattctctncataactcagtgtagcactttaaagtctgaaggacagccagat
3' EST: DT395326 [+]ggtatttttgtttgtatatctatgttatggccaattgtaccagcgcagaaataggagaggtgagaacactccaggaaaagaacctatcagctctctactt
ttagatcacacatagaaagtagtaagacagacatgaacatgaagagtgtggggaactgccaggaagaacagggaggcaagggctcagctgatatacatat
atattatctcagtattaccggtcacgctgcctgcagtaatgcgtatccgaaaattgttagatatcaatcaaccgggcaataagtgaacccacattggcca
acaagcagtcagtgtttccatcatggcaccatcatgacggttaaatcaatatagtggagtttaatctgaataatccttggcattcaccaagaagggtgaa
atgtagtgaactgccaggtatcagagcactaaataagggcccggtcgctatgtgcgcagctgagtgattctcttgccagtaaagtctccaaaaagcaaga
tacacacagtgccttgtaatagaataacatgtataaattaaacagacaaagtttcctgtgttacaaaaaaaaagtaaatgagattaacaggaagcaacga
cggcgttgtaccagttaatacacaatcctcacaggaaagcaacgcgttgagcggacacccagttacaaagtatacattctcctaccatatataaaactgt
gtgatctttgcttgtcatgtaacaaatggtgtacattctgcttggcacttgtactctagttttgtttgactcaagtatattaaattaaagcccacctaat
gactacatagaaagctatatatatagatttttcttgttataaaagatgaaggaggaaaaaaaaataaataaaaagccaact
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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