XB-CLONE-3178436
Clone Name: IMAGE:7854136
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Gene:
ybx1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT395502 [+]
caccgccggcctgagttaccatcaacaccccggggggaagttaaagcgagccaaccgccggcccgagtcaccacctccgaaagcagccaacttacaccat
caaagacacaatgagcagcgaggttgaaacacaacagcagcagacagtcgcattggagggcaaggccggccaggaaccggcggccaccgtgggggagaag
aaggtcatcgcaaccaaggttttgggtacggtcaaatggtttaatgtgcgcaatggttacggcttcattaacaggaatgacaccaaggaagatgtgtttg
tacaccaaactgccatcaagaagaataaccctaggaagtaccttcgcagtgtgggagatggtgaaactgttgagtttgatgtagtggagggtgaaaaggg
tgcagaggcagctaatgtaactggtccagggggtgttccagtccaaggcagcaaatatgcagcagaccgtaatcatttcaggcgctatcagcgtcgcaga
ggtcctccacgtaactaccagcaaaattaccaaaataatgaaagtggagagaagacagaagggaatgagagtgcacctgaaggagaggggggttcaaatc
agcaacgtccataccgcaggagacgtgttccaccatactacttgcggagaccatatgggcgcagaccacaatactcaaatgctcctattcagggagaagg
tgcagagggatcagatggtcaaggttcttcacaaggtgatcaaggtagacctgtgcgacagaatatgtacagaggcttcagaccacgatttcgcagggga
ccaccacggcagagacaacccagagaagaaggaaatgaaagaggac
3' EST: DT395501 [+]tttttttttgttgagacctttattaacagatgcttgcagtttgttgactcttgaaaaaaattaggtgtacgcttattacaaattaaaaatgaggttctta
aaaatcttagtttgaccagataggaaacaatttaaaaacctttgaaaggtacattgagaaaaaatatgtttttaaaaaattttcattcccaaaaggagct
tttagaaaaaaaaacataaaaaccatctctgcatttacccatgcaggatgaagttatctggtcaagagcaaaaagcaagcacttaaggtcttcagttcca
attctttgttcatttcttattgctggaatttctgtcttgacattttgatgacaaaaaaccggatggtggtagagttggtaagccggtatttactcagccc
cgccctgctcagcctcgggagcggacgtgttctcagctgatgtttcggctgccttggtctctttaccatcttgtggtttagggttttctgggcgtctgcg
tctgtagttaaagttacggcgataccgacgttgaggtgcctgctgactctgggtttcatccccctgattttctttgtcctcttcatttccttcttctctg
ggttgtctctgccgtggtggtcccctgcgaaatcgtggtctgaagcctctgtacatattctgtcgcacaggtctaccttgatcaccttgtgaagaacctt
gaccatctgatccctctgcaccttctccctgaataggagcatttgagtattgtggtctgcgcccatatggtctccgcaagtagtatggtggaacacgtct
cctgcggtatggacgttgctgatttgaacccccctctccttcaggtgcactctcattcccttctgtcttctctccacttttcatattttggtaattttgc
t
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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