XB-CLONE-3178392
Clone Name: IMAGE:7854092
|
Gene:
slc25a3
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT395531 [+]
taagtttaaattacataaatgcaataactctcatctgtttatttcaacagataaatacagcggtaaaaccaagttacaatgtgtaggctatgcggcaagg
tgagagcacaaccaataattacatctgaaacaaccataggtcctattttttagttgaatttataaaaaacgccttttgaaaatgttggagtttgcatgat
ttgagtccatgtttactgagtgagaccaagcttcttcttcaaggattctggcatctcagggggaggaggacgaggaagtctgaagtaaaccttcacagag
tcatagataaaccactgcaaagcagtcaaggtaccaatcatgatgatacgggcagtcaaacccttccagacacctttaggcccaagtctcttcagaactt
ccaaagcagtgcttcccttttccttgttcagtacagagaccacagaatccgcaggatgtgagacaatggcacagaagacaccagcaatatatccagccac
gaaagtgacaaccaactgctcagattttgaacattcgcttctgggtttgggcacaacatacttatagagagcttcaacagttcgttcaaagcaagcaaat
ttcatcattgtgtatggaatctgcctcatccaaagaggagcaacacctttgtaaaacgcccagatgccttcctcagcatacatcttgggagcagcttgtc
ttaaagtattagcatagcctggttgtgtctgaatgcgtacttttgcagcttccattggagccagagcaatatcagcaaagaactcagcactggcagacgc
agccaggtaaagagaagtacgccacatgtaggaattttcctcgcccagcatattagcatacagtatctttgacacttcatagaaaccaaacttg
3' EST: DT395530 [+]aatgtattcctccgttgtgcagttagcgcggaccaaccctttcaatgctccccactttcagttggggcaagattctgtaatatcaaggaaaggcaatatc
ccccaaacaaagccgacccgtcagcttgctgctgctgctgttcaagaagaggagtatagctgtgcttacggatctggcaagttctttgcactttgtggct
ttggtggaatcataagttgcggtacaacccatactgcagtggtcccattagatctggttaaatgcaggattcaggttgaccctcaaaaatacaagagcat
cttcaatggcttttccattacccttaaagaagatggtgttcgtggccttgcaaagggatgggctccaacgttcattggatattcaatgcagggcctgtgc
aagtttggtttctatgaagtgttcaagatactgtatgctaatatgctgggcgaggaaaattcctacatgtggcgtacttctctttacctggctgcgtctg
ccagtgctgagttctttgctgatattgctctggctccaatggaagctgcaaaagtacgcattcagacacaaccaggctatgctaatactttaagacaagc
tgctcccaagatgtatgctgaggaaggcatctgggcgttttacaaaggtgttgctcctctttggatgaggcagattccatacacaatgatgaaatttgct
tgctttgaacgaactgttgaagctctctataagtatgttgtgcccaaacccagaagcgaatgttcaaaatctgagcagttggttgtcactttcgtggctg
gatatattgctggtgtcttctgtgccattgtctcacatcctgcggattctgtggtctctgtactgaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: