XB-CLONE-3178383
Clone Name: IMAGE:7853902
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Gene:
cdk16
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT395537 [+]
cctgaaccgactcactgactccccaacccgggatatcggccccaacggtacgcgatcaggggaaccatggatcggatgaagaagatcaagcgccagctgt
ccatgaccctgcggggtgggcgggccacagagaagacatccaatgagcattcagagccaataacgggggaggagaatagcagcagtgataatgagcccat
cgtcagaaatgggagaagcctctcgtcccacagcatgcaatccttcctgcaccactacaccgccagctcctttcaaaagccgcccatcccccggcctcac
agcgccgtgtgccgcagcctgagctcttacctgaatcggaccagcagactagagatcgtccacgaagacttgaagatggggtcggatggagagagcgacc
aagcgtccggctcctcggatgaggttcagtcgccggtgcgagtgcgaatgcggaacaacgccgcgcggaagatatcaacggaggacatcaacaagcgcct
gtccctccccgccgatatccgcctccccgaggggtacctggagaagctggcgcttagtagcccccccttcgacaaacccctgagccggcggctgcggcga
gtctccctgtcggaaatcggctttgggaagctggagacctacatcaagctggacaaactgggagagggcacctacgccacggtgtacaaggggcgcagca
aactcaccgagaacctggtggccctgaaggagattcggctggaacatgaggagggggcaccatgcacagctattcgggaagtttctcttctgaaggacct
ganacacgccaacatcgtcaccctgcacgatatcatacacaccgagaggactttgacgctggtcttcgagtatttggataaag
3' EST: DT395536 [+]ggcttttggagttttattatttgttcatacagttgatatgcagcagcaggaggaggatggttggacctcttggctcctcccctgggtgataatagtaacc
gtattataacttattggggaagaagggcggcccccgtgggccggagtaactgcggtcaccccaccccctcactcagcatcgttcttctcagagacccatt
ggtcgttcttgcacacgtgtatgtgccccaatgcccccgcccaccggctgactccatactgtatctaagggtaactaacccacacctgcctaaaatactg
agctctgccgccggtccagtccggcttctttagtcctgagaaataaaggaacttgcttggagatgccccacgtcgtccagcggcagagaatgacccatcc
ttcgccccgttgctgccgccattacccgaaagctgccaaccgtctgccagccaatgagatggtttgggttcttggtgttcctctgatcagggcggggcca
ttaatgacgccgtctcttgggcctcgatgcaagagtagaggaggagctcacgagggagagctgggaaaggaggcggagcagggggtgtggcaggagtccc
tgggtggggacagcagcactgtgggtaacattgggggagataatatgggaactgtagatgccacatgctggcacggcctaatatcctcattcacttgccc
ccggctgtgggcacagagagcgggtgaggctctgcccggcgtcgcttcttcttcttcttccagccatctggatcatagtcttctaaatggcgcccaaagc
ccaaggacttcacccgagga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: