XB-CLONE-3178365
Clone Name: IMAGE:7854190
|
Gene:
spon2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DT395549 [+]
aaaatctagagtgcaaaccagaagagaaataactaaatttacagatgtagaagaagaaacagagtcactacaagtcacaaggcatatgcgtgaaacatca
ccagaggtttctaaattgcttggaagaagaaggtctctttcgccaacctatatagaattaatgcgtcctgtatctgaactaatcagaccacgttcaaagc
cagctgaggtgcgtaaacctgaaagacggtccccaacacctgaaagaacaaggccacggtcacctagcccagtttctagtgaacggtctctttcccgttt
tgagagaacagcgaggtttgatatattttcaagatatgaatctatgaaagcagctctgaaaactcaaaagacatctgaaagaaaatatgaagtattgact
cagcagcctttcacgctagaccatgctcctcgtataacattacgaatgcgttctcaccgcgtgcctgttggccaaaacaccaggtttatactgaatgttc
agtcaaaaccaacaggtgaagtgacatgttatcacaatgctgttgaaatccaggagagtagcaagattcatataagtaatgtaagtggtgtcgtaacgct
agaaattattgattgccagccagaagacagtggaacataccgtgtagtgtgctcaaactacaaaggggaaacctctgactatgcaacattagacattgcc
ggaggagatttttcatcatatgtgcctaagcgcagagatgatattgttcctaagtcagcctttcctgaattattaaagtcagaagactatgccgtctcat
ctttcaagaaaactccgcaatggaagctagctctgcaagaagagaggntagttcacaagtaacatctt
3' EST: DT395548 [+]atggtgcagaaaacatgctttattgcaatcaaagagctatcttcgggaggttcaataaataataatgctacaataacataaaataaaacaataaataatg
acgaagtgaagggcattcggaagttcttaacaacagcctgaaaagctgcggtgcatcactttattgccctgttttctcaacatataatatagtccctatt
ctaccacacacaatcactgcacccgataaacctcctgaagttggcactgattctgcacttgagtaccttaacctgctttgttgagggtatggccaggtga
tgttactttaaagcaagctgagggagtcaatgtgtcagaaaaagtgcagagggttttttatgaacaaagtgctggccgcttgcactttgaacatacaaaa
gttttgtctctgtaaaattgtaaatttccattatctgtaaataatgtaaatatgtaggtgttgcctgcaggagggtagttgcaagcagcacctaatattc
acagtaattcaatccaaatacttggatttcgtatagttcgtgtagagcatcctctcttgggtgtaggtttgtcttcaacagggagtttctcaacctgctg
aggtttgcttagacatcaacgtagagttgaggcacatagtttaatggcaggcaaggtattcgctccctaggaaactttctcctgcccaccgttggttcca
gtttcattttaaaacgtcataaaatatatggtacagaactccataaccggtaaactcggctatatacagtatgtacatgtcagttaaaaatgtatcaatt
tccctattcaataggaatatgtgatagcgtaaatcttttcgactttgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: