XB-CLONE-3178298
Clone Name: IMAGE:7854148
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Gene:
polb
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT395593 [+]
cacagtttagtgaaagcccacagagacatgccaccctgagcctataatatggctgaaatgctcagtggggcttcactggaatgattttctttctgtattg
taggttatgggcttccacgtgatattgtcacacaagcttttggttacatgcattgcaattataaggcaactaagggtgtcgtaagcaaacagcaggttaa
ggagagatgacgtggataggaaattggcatcagcagcagaagacaggggacataatatctactctcacccattgagttggtagaagtgttgggcagaatt
agacacaaaggaggggttgaaagctgtgaagtagaagctgtgactgctgatttgatcctcagatcagatataacatgcttctctcttgctttctagggtg
tgtgccagctgccttgtgagggggacgaggagcatccacaccggaggttggatatcaggctgataccaaaggaccagtattactgtggggttctctattt
taccggcagtgacattttcaacaagaatatgcgtgcccacgccctggagaaaggcttcactctgaatgaatacacccttcgcccactgggagtcacaggt
atagccggtgagcctcttccaatagacagcgagaaggatatatttgactatatccagtggaaataccgggagccgaaggatcgcagcgaatgagattgac
gttgggggttattttctttttaacccataagatacctgtttgccagcaagtctacaggtgctttgaataggccatttgctaaatacagtgacgtacaaac
tctaatggaggagcaaatcatgtgatactaggtgtgtgtgtgtgtttgtgctgtgggtgtgagtaggtgcagagctctataccaggctacgcaaccagtg
cccctctagc
3' EST: DT395592 [+]ctaatatttattaagcagtccagcttaatgcacatgattacattcttttcatagccccccaatactgcaacatctcattacttgtaaacaaaggttgttg
gggggggagtaaaacgttaactgctcaatcacaaaagcccctcattctttaaggcccagctttaccttggttccatatttcaagttgttggactacagct
cccagcacgctttaaccgttaattagcagtccaacatctgggaaccccaagagaagtaactgtgctataaaggggcttctcatgcagcccacctataggc
cctgcaacctacgcctgcttatcagctgtatatgactggtttcagtggcttcccacatagactaagcagggcacacatggtaagatctttaccaagccgg
tgggccaaatcagaattacacgatcttaccaagcacagccagtgtaaaccatcaggaaaagacagcattaatgcaccgacaaagtctttgtcttaaaaga
tatgcaaacctgcgcaattgttatctggctgtaaatggcatggcctgctgggggcctccatacttgggccaataagccacctgacttggtctcaaggggc
cgagttggcagcttttatcagactgtgtatgggcagcttttgggagttggctttgcttttcagaccagatctagcctatactgtgggcccagtggggcaa
taaataataatgagcagttgcaaggaaacatgtcttattctctttatatctgtctaattgggttacttaagccagaacttccatctaaagggccgtgggc
tgtatgtgtctacacagagagaccataaaacacgtgcctctgtaatattctgatgatatgctttcataaacacacaaatatcaatttaaataaatgtaac
agcaatacaaatgcttaaaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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