XB-CLONE-3178292
Clone Name: IMAGE:7853862
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Gene:
prkag2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT395597 [+]
tatatacgcgcttcatgaaatcccacaagtgttacgacattgtcccaaccagctccaaactcgttgtattcgacactacgttacaagtaaagaaggcttt
ttttgccttggtagccaatggcgtgcgagctgctcctctatgggaaacaaaaaaccagagttttgtaggtatgttaacaattacagatttcattaatata
ttgcatcgatattacaagtctcccatggtacagatctatgagctggaagagcacaagatcgagacatggagggagctgtatttacaggagacgttcaagc
ctctagtgaacatatttccagatgcaagtctcttcgacgctgtatattcattgataaaaaataaaatccacagattgcctgttattgacccagtcagtgg
aaatgcactttatatacttacccacaaaagaatcctcaagttccttcagctttttgtgtcagaaatgccaaagcctgccttcatgaaacaaaatttagag
gagctggggatcggcacataccacaacattgccttcatccaccctcacactcctatcattaaagctttgaatatattcgtggagaggagagtctcggcgc
tgccggtggtgggcgagtccggaaaagttgtagatatttattccaaatttgatgtaattaaccttgctgctgagaaaacctataataatcttgacatcac
cgtgacgcaggccctagagcaccgatcgcagtatttcgaaggcgtggtgaagtgcagtaagctggagacactggagacgatcgtggatcggatagtgaag
gcagaggtgcacagactggtagttgtagatgaagcagacagcattgtgggaatcatctccctgtcagacattctacaggccttaatgctctctccagcaa
gtgccaaaagg
3' EST: DT395596 [+]taatgatatggcaaaaagctcttaccgttcaccagaactgtgtaaaaattaaaaaaaaaaaaaaaaaaaaaggttttggggttttttgtaatttttttat
ttaaagtaaaaataaataatgattattattgttcagtgtctcatatatttgggctctaaatcaaacaagcaacctggcgtcaggtcgcacctattgagcc
aacgctgccatcgcccgtgtatgtatgtatgtatgtatccaatgcctgacagtaagcggagagaggcgtagcagtgcccccttgatgtgagccccagaca
ctacagccatggtgcaatgaatggcgaggggggcggggcttttgcaggcaaccgcaggatacactaaataagaggcgggcaagtttacatgtggctttag
ttggcagccctttcacattcagtacagtgtcgctttgcacccaccggttccccacccgctgacagtgctgggcatcgtatcccataatgcaaagttcttt
tggactgatacagagcactatgctatgcgcatacagcttattaggtaacatgacaatcgtaacgtcagacatgaagcgctagggcctcattttcaatatg
tcaaaaattattttttgaaaaaaagtacaaccgtttcccaccaataaatgagctcgtgaccacgctggaccggtacaagagcccgctgacaccgtcagtt
gcacattcctgtccagttgtgttcctctgttgcattcagggtttctgaggcaaaagattgacgcaaaaaaaacatcattcaagttcttcttttctttaaa
aaattctatatattatatatatatatatatttatatgttctgcattcacggncattctgcttcactctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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