XB-CLONE-3178114
Clone Name: IMAGE:7854164
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Gene:
eef2.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT395715 [+]
tgaaaccgtgcttcgtcaagccattgcagagcgcatccgccctgtgttgatgatgaacaaaatggatcgtgctcttttggagctgcagttggaaccagag
gagctctaccaaacttttcagcgcattgtggagaatgttaatgtgattatttccacctacggtgaaggagagactggacccatgggaaatatcatgattg
accctgttcttggcacagttggttttggctctggtctgcatggctgggctttcacactaaaacagtttgcagagatgtatgtcgcaaaatttgcagcaaa
gggggaaggtcagttggctcctgcagaacgggccaaaaaagtggaggacatgatgaagaagctgtggggtgataggtattttgaccctgcaaatggaaag
ttcagcaagtctgcaacaaacgctgatggcaaaaaacttcctagaaccttctgccagcttgtattggatccaattttcaaggtatttgatgcaatcatga
attttaagaaagaagaaactgccaaattggtcgagaaactagacatcaaacttgacagtgaggacaaagacaaagagggaaaacagttgcttaaggctgt
tatgagacgctggctcccagctggagaggctcttctgcagatgattaccatccacttgccctcaccagtgactgctcagaaataccgttgtgagctgttg
tatgagggtcccccagatgatgaagctgccatgggtatcaagaactgtgaccccaaaggccctcttatgatgtacatctcaaaaatggtgcccaccactg
acaagggtagattttatgcctttggaagagttttctcaggtgtagtctccactggcctcaaagtcagg
3' EST: DT395714 [+]aaatttttttaatggcaccaataagtcattcaggttattgcagtacatctttgctctacattctgccctcgttcctgtcaaccctcaaattcacatttac
ttccccagccatccaagatgttttcagagagcagaagctacccatcaagaataaatagaaagttatgatcatgccaatgaaagattatattcagggttcc
aacagaatgttgcagggaatgctgacatcttttgggtatagttcatttccacttaatcaatgctgacaggagtaaagcaaaggtttacagcttgtcaagg
aagttatccaaggcttgaacaccctctttcaagcccttcctcttccgggtctctgccaccacttggaaaggcctggtggagttatcgaaaggatctccag
gtagaatttgccagtggtcaaacacacactgagggaaggcctggccaccagtgtttgatcttaagtcagcagtgaaaccaaatgactcatttacaggcag
gtatgccttaaccacaaacattggtgttccagccacctgggattcttcaaagacatgcccacgctttctgttcaagacaccatagatacctcctaccacc
tgctcagggcactgaatttcaacaaggtagattggttccatcagacgtggctgagcagtcaacacacaagcatacaagacgcggcgtgcagttggaatga
tctgtcctcctcctctatggatggcatcagcatgaagtgttacatcatggacatcaaaacgaacacctctcaaattttcttcacacaggacaccctcctt
tgtagcccactggaatccctgcacaacgctgtctttgatttcattcagatactgaactcccttagtaacatcagtaagtatgttgggcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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