XB-CLONE-3177894
Clone Name: IMAGE:7854471
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Gene:
tmem120b
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT395860 [+]
ctaacatgtcgctacagaagtgccaggaagaatggggagagctggagaaagaattccagcagctacaggaaacacacaaggtatacaaacagaagctgga
agaattgaatggccttcagaatctatgcagcagttatattaataagcataagaggcgtctgactgagttaaaaggcaaccttcatggatataaacatacc
tctaatgttgaagaaaaggaactcgtccagcaaataaacagcacaattaaagagaggcacaatgcatttttcgacatggaagcctatttgccaaagaaaa
atggtttgtatttaaacctggtgcttggaaatgtgaatgtcactctccttagtaatcaagcaaaatttgcttacaaggatgaatatgaaaaattcaagct
ttacttgactattatattgttactgggagctatcacatgtagatttgtgcttcattacagggtgacggatgaagtatttaatttcttattagtctggtac
tactgcactctgaccattagggaaagtatcctcatcagcaatggttcaaggataaagggatggtgggtatcccatcattatgtctccacgtttctttcgg
gagtgatgttaacatggccagatggactcatgtatcaaatatttcgaaatcagtttctggcattttccatatatcanagttgtgtacagtttcttcaata
ttattatcagagcggctgtttatatcgattacgtgctttaggagagagaaatcatttagacctcactgttgagggctttcaatcatggatgtggcgaggc
cttacatttctacttcctttccttttttttggacatttttggcagctgtacaatgcaattacactctttgggctgtcaagacatga
3' EST: DT395859 [+]tttttttcacagtagtaaacatttatttttcattccctccagcctgtgcccttactgtacttccatataaacatggtaccaaccataacacactggtttg
ggaaacccacctttgcatatgtgattcaaaatgcaatgtggccaaaatgattaatggggattagtagtagagtcagtaattccattagcagccgtagaac
acagtggtctgtaccgcaggcctgagcgtatcctttccagaggggggaaaaaagcagcttagaaatggcaagtacgaaagtctctcccagagggaaactg
gccggcatctatgcacccaccttccaatccattataccaacccccactgtaatagcctgatcagggacccaaagcacagcccctgcactttccagcccac
cctcatgctaaagtccacgatgggggaggggcaaatattccatccagtatatttttgccccagtcttacaccaggctcaaggcttctttagcttgttctt
ctgaaattttgtgtgaacaacttttagagttgtcaggaagttccccaggaagagcaggagaaatgtgagagccaacacaaacacctgccattctttgcat
tcttcatgtcttgacagcccaaagagtgtaattgcattgtacagctgccaaaaatgtccaaaaaaaaggaaaggaagtagaaatgtaaggcctcgccaca
tccatgattgaaagccctcaacagtgaggtctaaatgatttctctctcctaaagcacgtaatcgatataaacagccgctctgataataatattgaagaaa
ctgtacacaactttgatatatggaaaatgccagaaactgatttcgaaatatttgatacatgagtccatctggccatgttaacatcactcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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