XB-CLONE-3177858
Clone Name: IMAGE:7854283
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Gene:
srpk2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT395884 [+]
catcattctctctcacacacggcttgtagtctgccctggattcacccatcatcccccactctcccatcctgcttgcagctgtctcttccctccgccccct
gtctctccccactgaggatgtctccttccctccccacttccttcccaactctgtcatgagctccaagaaagtttcaggtaacagccaagggaaaaagagt
aaaaagaagcagaggcggcagaggaaggctgcagagagagacgtgcctggagttctggaacctcggagtcccagtttagatagccagcctggcctcccag
agcatagcctgcaggatgtgcctttgtctcccggaccccctccaaccccacctactcctgtgctgcctccaccgggacccttaggctctgacaatgaaga
acaggaagaccctggagattactgccgagggggttattacccagtaaaaatcggtgacctcttcaatggaaggtaccatgttgtacggaagctgggctgg
ggccacttctccactgtctggctctgctgggacctacagaggaagcggtttgtagcactgaaggtagtgaagagtgctgtgcactatacagagacggctt
tggatgagattaaactgctcaaatgtgtcagggacagtgacccaagtgattcaaaacgtgaaatgattgtgcagcttattgatgacttcaagatctctgg
agtgaatggagttcatatctgcatggttctggaagtgctggggcatcagttacttaagtggatcattaaatctaattatgagggtgtaccactcccatgt
gtcaagagcatcttgcggcaggtattacagggcctggaatatctgcacaccaagtgtaaaatcatccacaccgacattagccagagaaca
3' EST: DT395883 [+]ctgatgtgaggggggtgcggagctgacagcagagccagatggtggaggcattccagactgctgccaaatggttgcctctgccgccagtcgccggatatat
ccttcctccacgcacatcaggatgttctctggcttaatgtcggtgtggatgattttacacttggtgtgcagataatccaggccctgtaatacctgccgca
agatgctcttgacacatgggagtggtacaccctcataattagatttaatgatccacttaagtaactgatgccccagcacttccagaaccatgcagatatg
aactccattcactccagagatcttgaagtcatcaataagctgcacaatcatttcacgttttgaatcacttgggtcactgtccctgacacatttgagcagt
ttaatctcatccaaagccgtctctgtatagtgcacagcactcttcactaccttcagtgctacaaaccgcttcctctgtaggtcccagcagagccagacag
tggagaagtggccccagcccagcttccgtacaacatggtaccttccattgaagaggtcaccgatttttactgggtaataaccccctcggcagtaatctcc
agggtcttcctgttcttcattgtcagagcctaagggtcccggtggaggcagcacaggagtaggtggggttggagggggtccgggagacaaaggcacatcc
tgcaggctatgctctgggaggccaggctggctatctaaactgggactccgaggttccagaactccaggcacgtctctctctgcagccttcctctgccgcc
tctgcttctttttactctttttcccttggctgttacctgaaactttcttggagctcatgac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: