XB-CLONE-3177802
Clone Name: IMAGE:7854479
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Gene:
srpk2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT395921 [+]
gatccatcgattcgatcatcccccactctcccatcctgcttgcagctgtctcttccctccgccccctgtctctccccactgaggatgtctccttccctcc
ccacttccttcccaactctgtcatgagctccaagaaagtttcaggtaacagccaagggaaaaagagtaaaaagaagcagaggcggcagaggaaggctgca
gagagagacgtgcctggagttccggaacctcggagtcccagtttagatagccagcctggcctcccagagcatagcctgcaggatgtgcctttgtctcccg
gaccccctccaaccccacctactcctgtgctgcctccaccgggacccttaggctctgacaatgaagaacaggaagaccctggagattactgccgaggggg
ttattacccagtaaaaatcggtgacctcttcaatggaaggtaccatgttgtacggaagctgggctggggccacttctccactgtctggctctgctgggac
ctacagaggaagcggtttgtagcactgaaggtagtgaagagtgctgtgcactatacagagacggctttggatgagattaaactgctcaaatgtgtcaggg
acagtgacccaagtgattcaaaacgtgaaatgattgtgcagcttattgatgacttcaagatctctggagtgaatggagttcatatctgcatggttctgga
agtgctggggcatcagttacttaagtggatcattaaatctaattatgagggtgtaccactcccatgtgtcaagagcatcttgcggcaggtattacagggc
ctggattatctgcacaccaagtgtaaaatcatccacaccgacattaagccagagaacatcctgatgtgcgtgga
3' EST: DT395920 [+]gagccagactgacactctttatttttggggatactggtcttttaagtgggtgggggctcagcagnctttttaaatcctgagtgcttttacaatatctaaa
gtagaactctcacaccaagtatgtaaacatctctgcaatttagagatctctcttgcactgtaaatacacactttcatatcgccatatacctctcacaata
aaatatattcctttcttggggcaagaaaccctagcagtgtcctttttcctgacatttcatctctttagtgcaatggcagctggctgggtattgtagaaca
gagcgacaaccctgcttccagcacgctatagtagtacaccttccaccactgccatcatttgtgctaactgcaattctgttttgtgttatgtagtagtgca
gcaagccttcctccatccacttccccagcgccatttaccgacatacaaataacaatggccaaacgaatgccaactgtgtgaccttgtaaagaaaattagc
aactgaacagaaaaccctcttaatacaaggtaagaacctggtattgtagcagtctttcccaaccgtacacatatatatcacgctgttgttcctttggtac
caaagaatcccactgctttttaaaattttagatcaacatggcagttgagcaatatcaacaccgttgtgtaagttggacttaacatacaaaaacttgtcgg
aggcttctatttgcaacagaaccagtgggatgtttcctgtaatttcacaagaatttttttgttttttaaagtctttttcacgataaacgcagtaatgggg
g
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: