XB-CLONE-3177677
Clone Name: IMAGE:7854347
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Gene:
nrap
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396004 [+]
caccgggcacggctacaggacgctgctccatcactacagctccctccccaccgacatgaaagtggaatgggccaagcaggcctacaaccttcagagcgac
aaccaatataagtccgacctgcggtggatgaggggagtcggctggatgacggcaggttcgttggacgcggtacaggccaagaaagcagcggcgttggcca
gcgagaaacggtaccggcagtcgccctcggcgctgaagttcaccagcattaaggacacccccgagatggtccaagcgaggatcagttataaccaggctgt
cgacagtaaatacaaggaacaagggaagagcgtgaagcaccattacacccccactgccgaactgccggaaatcctacaggcaaaactgaatgccatgaac
ctgagcgagacgagatacaaggagtcgtggagccgactgagagacgggggctacaaactcaaactggacgccattcctttccaggcggccaaggcgtcca
atgagatcataagcgaccacaagtacaaggaggagtttgagaaggtgaaagggaagatgattggccttcgccggctggaggatgatataaatatcgctca
ttccgtccgtgccgcctccctgctcagtgatgttcagtaccggaagggctttgagacgactaagacgcagttcaagctgccgatggacatgatacacctg
acccatgccaagagagcccagtcgttggccagtgggcaggactataggcacgcgctgcaccattacaccgccctgtccgatgacgtgaggctgctggcgg
ctaagaaggcgcatgaactccagagcgagaaactctaccgctcccgacctgacttcatgagaggggtggggtgcatcac
3' EST: DT396003 [+]cgaatggagtcgcgtttattccgtacaaacgattctacaaagaagcccaaatctcgtgtataaaaactgatcctgcagtcgctcgagctccgatatacgt
gacaaacaaacacaggggggtgtcggctctgcaaggtcagactcaccggcgatcccggtacaatggaaacactaccagtcaaggaaatgctattaaacta
cactgggggggcaattaaatgtaaagaaaccaaggggggcagtttatatgtggcattaacagcataactgactagttccccactagtaatactattatat
agaacccccacacattctaccacattctgcctcctgcggtggcccaaagtagggcaaagcagggggggcagtttaggacgttccttgtgcacgggggcag
aataacccaggcagtcatgataaataccctgagttacacacaaacatagcaggagctgccccgttcctttctatctgccccggggaattcccaagcgatc
ggaccggcaccaaatgttcattttttttttgccagaattttcttcctcttcacctgaaggatctcggaggcatcgggattaacatcgctgtgtggggact
ggtaccccgtctcgtctgctgaataaggagacactgacgcccggtctgactctgtgccatctccgtatgacagttgggccgcccggcgagccagttctac
tttgtagtagccagggggggtccaaccaacccctttcatccagttcagatctgatttgtacttaatatcgctctgcaggctgtgggcttgcttggcgtgt
gtcatggctagctgctgggggtcacacgtgtagcggtgcaagtgctgccggtagtggatgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: