XB-CLONE-3177668
Clone Name: IMAGE:7854539
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Gene:
ahsa1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396010 [+]
tgaagtgtaaaccatagagagaggctagtggcagggaaaggagcaaagcacaatatagcagagcggtgacaggcagtggtgcagacggcaaagacacgct
gattgaggcctttctcgcagcggcgctgaaggtaccagaatagtttcttgcatttagatcttcacttgtttagccatggcgaagtggggagagggggatc
cgcggtggatcgttgagatgagagcagatgccaccaacgttaacaattggcactggactgaaagagatgccactagctggtcctcagagaaaattaaaga
actaatgatggacataagagtggaaagtgaggaagggacatgtgaaataacagaagtaagcaagctggagggggaagcatctatcaacaaccgaaaggga
aagctcattttcttctatgagtgggatatcaagctgaactggacaggggtgtcaaagtctggggtaaaatacaaaggacatgttgagattccaaatcttt
cagatgaaaatgatcccagtgaagtagagatcagagtgtccatggctaaggacgagcctgaaactaaccttattggtgtaatgagaaggcagggaagcaa
ccaaataagggaagctgtggctctgtacatatccatgctaaaaacagagtttacacaaggaatgatccttcccacagcaaatggtgtttcacatagcaga
gttacagagataaagcagaaagctgaaacaaacatgccacagactagtaaaagccagacctgtcagaatgcaggggttaaaatacctacttgtaaagtga
acataaaagacaccttcctgacttcacctgaaagattgtacagagtgctc
3' EST: DT396009 [+]ctgggctccgtctgacatgtacactttttttttgttggaacaatacactgtacaaagccaccatcagacacaagaaatcatttcccagaaatcaaatgct
cagagtctcttaccacatggagataaaacccccacaaattaaatatttttcagtccacaagacaatatttaattatttatagaccagtaaaaaaagaaaa
aaaaaaaccctctgtccaccccccagcaaaatttataagagcaaagctccatatccaaatgtctgctttatgccatcaaaataatatcttttccagcctt
ctttggtacgatcctcttcaccctgtgggacaccccgagcttccatttgcagctctgtctccccacctttgtctgtgaaagtcagagtgatggaagcatg
atgaccttgtggccaagatttaaacctccaagacattacaatttttttctcgggttccaattctaaaaattctccactaacatttcctcccagaagctgg
aacttcccacctttgtctgctgttaaagatgcaggggcatgtgtaaatccttgcactagctcttgccgggtgagcactctgtacaattcttcaggtgaag
tcaggaaggtgtcttttatgttcactttacaagtaggtattttaacccctgcattctgacaggtctggcttttactagtctgtggcatgtttgtttcagc
tttctgctttatctctgtaactctgctatgtgaaacaccatttgctgtgggaaggatcattccttgtgtaaactctgtttttagcatggatatgtacaga
gccacagcttcccttatttggttgcttccctgccttctcattacaccaataaggttagtttcaggctcgtccttagccatggacactctgatctctactt
cactgggatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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