XB-CLONE-3177630
Clone Name: IMAGE:7854399
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Gene:
mustn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396035 [+]
cacagggtgccccagttaagaagaagaggcctgtgatgaaggaggaagaccttaaaggagcaaaacacaagctggcccaccaggtcccaatcaaatccaa
aacataccaggttatgcaggagtgtgaacaatctggaacaacagccccttctgtgttcagccagaacaggacaggcggagaaacagcctttgacaagcca
aaggaaggcccagcgaaaagtgtctttggttaatggcaaaagaacattcaaataactcggctgcccagcaagtcatttttgttcccaccttctactcatt
gtgctgcgcacatcatgcagagtcttacactatatccgcgccagtcatttcactgtctgttatctgtttctaagttactgtaagactttatttcattgct
cctatggtcgggctgtgctgcattatctgatatactttcagtagagacccatttatatgttttctaaactggctcttcagttattaagtcaacaaaggag
ccaaatatgattttttagatttcctatatataaaatggaggaagtgaatacagaatttgtattttctaaaatccaaacccttacaggtccttaggctttg
gctggaccaaatcagaacacctcttttcgtcatgtgactttactgggctggggacctaaacacagtcatatcacctactccccatcatcagggtgatatg
gttcagtcctggcatttttcctatgtcaacatattaaggtggattcaggactagaccatttcctgcctggtgagggatcacctgcttttattgttgcatt
anatgtaaaattaggacttttcccgtttagtactcatccctaacaaactaagggctgataatctgagcaaccttt
3' EST: DT396034 [+]cgcttgtacagactttgttcacaagtttaattgtaaaaccaggaaagtttaaaaaaaaaaaaaccatataagaacatagcgacacaagaatgatcagggc
taatacacaacacacatttacttctcagtgatgctgtgactgggtgcactgttctacaaggtttcatttaatacaccctgacactatctagcaccccgag
cagaagcctagtgtgatgccagtggcactgagttactacaaggactaagcctgaatgtgtggaagggtgaataggttggggccttatacactgaccaatc
atagaactaactggaacacagtaaatctctcttgccaagtcgagctgtcgctgctggtcaatagaccaatcataactaattgctcctacaaggtcattca
cttattgaatagaaggctacaaggtcaactttctctttattctgtcttctaatatcattactggctacttgagatatggacgccgtttttattctacagt
tggagcctataaaggttgctcagattatcagcccttagtttgttagggatgagtacctaacgggaaaagtcctaattttacatttaatgcaacaattaaa
gcaggtgatccctcaccaggcaggaaatggtctagtcctgaatccaccttaatatgttgacataggaaaaatgccaggactgaaccatatcaccctgatg
atggggagtaggtgatatgactgtgtttaggtccccagcccagtaaagtcacatgacgaaaagaggtgttctgatttggtccagccaaagcctaaggacc
tgtaagggtttggat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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