XB-CLONE-3177440
Clone Name: IMAGE:7854370
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Gene:
otud1
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396161 [+]
ccgaggctcacacacacctcctcctcctctaacatgcccgccttctcctgcctccccagcgctgcgctcggcgctcacacccacttagagtccatgtgca
ccccggagaggatagtgcccgtccgcctcctgcagcgaagccctaacgggcagcagcgctacatctccgaagccacaaatgcacctgtctgcgccccagg
agcaagccctagcccccgcccagcagccccaggatatccatgcatggctcagcgacatggtgcaggagacgatccccaggcccgacaccatcgcggctcc
catagaagatcaatgctacgaaagtaacagcagcagcagcggcggcttttggcacccggaaagcccggagacgacggccgcacaatcgcaggccggtaac
agggagcggttggccttacacctggccgagctggaaaagcaggacaagtacctgcgggaggagcagcgctatcgcttccatatcatcccggacggaaact
gcctgtaccgggccgtgtgcaaggcgctatacggggagcaaagcctacacgccgagctgcgggagcgcaccgtgcactacgtggccgatcatctagacat
tttcaacctcatcatcgagggcgacattggagaattcctcatcaacgccgcccaagatggggcctgggcgggatacccggagctcctggccatgagtcac
atgctggaagtgaatatccgcctgaccaccgggggcaggcccgagtgccccaccgtctccaccatggttcatcggatcggaggcgaggatcccagccggc
ctagtatctggctaagctggctcagcaacggccactacgacgcagtattcgagcagcccctgccncacccagagtatgagcggtggtaccggcagaacaa
gcgcaaaggcagcgggacgaggaattg
3' EST: DT396160 [+]aatcagcagaggatagaaatcgccattcttacaaaatcattaagtataaacaacagtaataaatactgaaccgcaatggggagaaaaaaacaaatgtaaa
caaacattgtcatgtggcccataagtttcctacacacttggatctcaccagccaaaaagctaaagtccagtcatttccactataacaaaaacaaatgaac
acaaaatgttaacccttcatatagaaacaggtttgtaaataacaactgaaaatgtcttagatataaataccacatgtccatttattattaactggcaagc
ggaagtctttgtccattaaaatgatgttacaaaaaaatagttaaggcagaggcatccaacctggggccctgcaactttagctaaactacaactcccagaa
gccttgacagatgctggaaatcgttattggggggggggtgaaggtaggacatccctgagttaaggcatcaagtggtaaatgagaaggtttaaagtgcagc
aaacttccactggtgagagtccaggtatatacacaacacagtgggcctggcaactggcagtcagctggaacaaggcttggcacaccctgtgttgcatgga
ctgcagaacgtattaggaaacactgactggtacccaaagagtcagagaggccattggtagtgcctgtgatatttcctgctccagacactggcaatttgag
gcctttggtgtaagggtgccaagtgtgtccctttagaactgtgagcctaacgcaaagccccagctgcttgtaacgggcccggagtgcaaggccccagcac
cgctgtctcctgtgtttatccatcatggctgcgctttccacatgtgccagttttgca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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