XB-CLONE-3177333
Clone Name: IMAGE:7854284
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Gene:
ssb
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396232 [+]
aaaaggatatccgttcagcgattactggcaatcatccttgctgctagcatggaaacatatgtttgtattaacaaaaggtataaaaataaagtgcaggata
gcagtatacaggactgccatgcacccacaaaaataaagtaaacaaaaaaaaaaaataatctgtcattgcaggttgatatcgaagtggatttcattagatt
tgctttcaactgaccatctggatctggagtttaacttaaaaaaaaccaaaggcattacagtacattactgatccccagcttcagttttggattgtttcaa
tgcaggaccgtcatctgccttgtcatctagtgatctcttctttggactccctggagcttctgttccattttttggccctgccgattcctctgactttgca
gtcactttcgggggactcactgattctaaaacatggaacagcaaagaacattcagcaaacagacataagcagatatgaacaggaatttgactgctccttt
ttaccttccatatcatcctcatcactgctatcaaattttttcttctttccctggaattgtattctcttcctttgagaactgtcatttcctttgccgcctc
gtccttttcctttgaatttccttccatctctgcctttgcgtttgttgaaagactcctgtttatcttccataatttttttcagagcttctttctcagcatc
accctcaataagctcccactttacggccttgccctttaacttcaggtcgtcattgttggcagccatggccttgtccagtgcttttttagcattcatttta
aataatacaattccctctttagctcccctgctaaagtcaatccattcaatatctccatgagcctg
3' EST: DT396231 [+]gaaaatggctgaaaatggggacaaagaacaaaagttggatctggatacaaagatctgtgaacaggttgaatactattttggggaccataaccttcctaga
gacaagttcttaaatcagcagatattgctggatgatggctgggttccccttgaaaccatgatcaagttcaacaggttgagcaaactgacaaaagatttca
acaccatccttcaagcacttaaacaatctaagactgaactcttggaaatcgatgaagataaatgcaaaattcgaaggtctcctgctaagcccctccctga
attaaatgacgagtacaaaaattctctaaaaaacaaatctgtttatattaaaggatttccaaccagcgcaaatctagatgatatcaaagaatggctcaaa
gacaaaggtcctattgaaaatatccaaatgaggagaacactccagaaagaatttaagggctccatctttctcatttttaatacagaggatgaggcaaaga
agtttttggaagacagaaaccgaaagtacaatgacagtgatatgattatcctatcaaaggaagaatactttgcaaaaaagaatcaagagaggaagctaaa
taagtctgaagaaaaagccaaatcaaagcaggaaaatgaagaagcccagaaacaggctgaggatgcagaaaggaaactgatggaagaaagggttggccgc
ctcctgaagttctctggtgacttggacaatatgacatcaagagaagacctttcggctctgtttcaggctcatggagatattgaatggattgactttagca
ggngagctaaagagggaattgtattatttaaaatgaatgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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