XB-CLONE-3177291
Clone Name: IMAGE:7854240
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Gene:
capn3
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396260 [+]
caagtgcaaatcatatatcaacatgagagaagtctgtcagcgtttccgattgcccccaagtgaatacgtcattatcccgtctacctatgagccccaccag
gaaggagagttcattctgagagttttctctgagaaaagaaacatttcagaagaagttgaaaacaagatagaagcagaaaaaccaattaaaaagaaaaaga
aacccaagcctatcatctttgtatcggacagatcaaacagcaataaggagctgaatgtggatggcgccactgatgaagatcagaaaaaaatggacgcaga
gaaaaaagataagacctctgctagtacagacacagagactgaggaagaaaaacagttcaggaatatcttccaacaaattgcaggagatgacatggaaata
agtgctgatgagcttcaaagtgtcctgaacaatgtagttaacaaacataaaactctgaaatccagtggattcaccctagaatcatgccgcagtatgattg
cgcttatggatactgatggctgcgggagactcaacctgcaagaattctatcacctttggcaaaagatcaagcagtggcagaaaatctttttgcgttttga
ttctgaccagtcggggaccatcagtagctttgagatgcgcaatgccatcaatgaagctggtttccatctgaacaatcagctgtatgacattatcacaatg
cgctatgccaataagagaatggaccttgactttgacagtttcatctgctgctttgtgcgcctggaaggaatgttcagggcattccatgcatttgacaaag
atggagatggtatcattaagctgaatgtgctggagtggctgcagcttacca
3' EST: DT396259 [+]ttttacacacccaaagttatatttaaaaagactttaaaacagcattgcttttaaaagagcaatataaatcacgctatagatatatagttctccaacataa
cccttctttaaaacaacatccaggcatggactctgactaggaagccagttgctttcttggtgatttaaagcagaacacagttttagggtaaggcaatggg
gtatgaacatggaggactgtatcatgaggtaaatgtgacaagctctgtccaacagagatcctgagagactttcctgtgcagaaccttgagagaagattct
aatcaggagccaaaagttggtgttcaggccttctaagcatacatggtaagctgcagccactccagcacattcagcttaatgataccatctccatctttgt
caaatgcatggaatgccctgaacattccttccaggcgcacaaagcagcagatgaaactgtcaaagtcaaggtccattctcttattggcatagcgcattgt
gataatgtcatacagctgattgttcagatggaaaccagcttcattgatggcattgcgcatctcaaagctactgatggtccccgactggtcagaatcaaaa
cgcaaaaagattttctgccactgcttgatcttttgccaaaggtgatagaattcttgcaggttgagtctcccgcagccatcagtatccataagcgcaatca
tactgcggcatgattctagggtgaatccactggatttcagagttttatgtttgttaactacattgttcaggacactttgaagctcatcagcacttatttc
catgtcatctcctgcaatttgttggaagatattcctgaactgtttttcttcctcag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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