XB-CLONE-3177182
Clone Name: IMAGE:7854536
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Gene:
ivns1abp
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396332 [+]
cccagctaaacattagaaggcaccagtctgctgtttgtgaactaggcaacaagatgtacattattggaggagctgaatcttggaattgtctaaattcagt
agaatgttacaatcctgaaaatgacacctggacattggtggcgcctatgaatgtggctcgacgtggtgctggagttgctgtttatgagggaaagctattt
gtggttggaggatttgatggtacccatgctctaagttgtgtcgaatcatatgaccccgaaagaaacgagtggaagatgatggggagtatgacctctgcaa
gaagcaatgcaggaatggttgctgtaggggatcaaatatatgctgcaggtggttttgatggcaatgaattcttaaatacaattgaagtttataaccctca
gacagaggagtggagtccatttacacacctgtgcaaatcctagtaatcctcatttctcatcagatctgcttgggcttaagtccctcatgtttgttgtacc
agcaacgtacgtttttgcatattgcatattttcaaaaagttgtaaatacttatttttcatttatttgtaagaaggaaaggttaaaataacttgtgctaac
tttgtaattactctactgtggaattctatgaaacacaggattttttttttttttgttcctttctgcaagcattcttgtgtatatgttcgctctgttccta
atgcactgacgtgtagagttccagttactgagtagttacaaaatcttacacggttttaattgaatgcaagtgatttattttctttagtataaaataatgt
gcttt
3' EST: DT396331 [+]catttacagtcaattttatttaataatgtacattagaagtggggaaaaaaaagttattgaatgttttttttcttccttgtttcgatgataaaatattgtt
ggtggttttcctttacagagtgcctacaaaaagattcagcttgatcaaagactctaaaaatgagtatttgcagacaaatctgtttctaaacaactggctt
cggtctctgcatttcaagacattcctttgtttaaagagtaaaaaatgcatttgaaaagactgcagctgtcttcctacatgaaagcaaaaatgatgctgtc
tatcaaaatacacagcttgaaggataaatacattaataaagtgttgacaattccttgtgcctgccactgagtatataaaacagtaataaaaaggcaatcc
aaaaggtgcgtgttttggcatacctgcacttctttactcaaaaatcttgattcagtaaggcttttaaagcacattattttatactaaagaaaataaatca
cttgcattcaattaaaaccgtgtaagattttgtaactactcagtaactggaactctacacgtcagtgcattaggaacagagcgaacatatacacaagaat
gcttgcagaaaggaacaaaaaaaaaaaaaatcctgtgtttcatagaattccacagtagagtaattacaaagttagcacaagttattttaacctttccttc
ttacaaataaatgaaaaataagtatttacaactttttgaaaatatgcaatatgcaaaaacgtacgttgctggtacaacaaacatgagggacttaagccca
agcagatctgatgagaaatgaggattactaggatttgcacaggtgtgtaaatggac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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