XB-CLONE-3177141
Clone Name: IMAGE:7854444
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Gene:
smyd2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DT396359 [+]
cccccctcccgcaccatgggacagcccgagggtctggagcggttcgacagcccagggaaaggccggggtctgaaggctacgcgtccattcgcgctggggg
agctgctgttcagctgcccggcctacacctacgtactgactgtaaacgaacgggaaaccactgcgagttctgcttcgccaggaaggaaggtttatcgaaa
tgcggcaagtgcaagcaagccttttactgcaacgtggactgccagaaaggagactggcccatgcacaagttggaatgttcggcgatgtgtacttacgggc
agaactggtgcccctcggaaaccgtcaggcttaccgcgaggatactggccaaacagaaaacccagacagagaggacggcatcggaaaggttcctatcggt
taaagattttgaatcccacctcagcaagctcgacaacgagaagctcgagttaatccagaacgacattgctgccttgcaccgtttctactccaagaatttg
cactattccgacaacgcggcgcaagtgttcctctttgcacaggtcaactgcaacggcttcaccattgaagatgaagaactctcccatttgggatccgcca
tattcccagatgttgcactaatgaatcacagctgctgccccaatgtgatcgtcacttacaagggaacggtggccgaagtcagagccgtgcaagagataca
cgccggagatgaggttttcaccagttacatcgacctgctctaccccacagaagatcgaaacgacagactgattgactcctatttctttaactgcgactgc
agagagtgctctactaaacagaaggatccggcaaaattggaaatc
3' EST: DT396358 [+]ggggttttttgttttttattatcacactgcagtgtaaaacagcaatctcggtgataaaattagcaggcaaactttggttgcattccattcgtaggcaacg
gaaaaacacggcattaaataaaaagtaaaacccgcgtccgggtttcaatcaaaccgtaaaccaagctcagaaatcaattgacgtgaaagttatgggcggc
tcctctggaaaccctgtttggcatcccagctccggtcgaggtgaaggaattcagagaaatatacagaacggccctttcattcaggataaaggccaagtga
gcggtggtagaaatattaagagttttacaagcaaacacatgaaaaagaaacccattgggcttcattgcataacgcagaaggactccaggaccatccctag
tctgtaggccaccaacttttcttgaacaaagagttcccctaagagtttcagtccaactaaagaggctgaatgcccctgtaggcacagcgttgccatcagg
gaactgaggtcaagccttgtagtccttggactaaaactcccatgattctcagcccatcatgaaagacagaggagttgatagtggtgtctcggtgtcctgg
atccccattccccgaagcatttatgaagggacttgcatagaatctgtgcctcacagctccaattcctttttgatctctgctatataatgatgatcaggtc
cgtgggctatgtccattatagctatggcctttttcagagcctttgtcccaatagctttcttttccaggcccatatacaggcggcccagcttcagccacat
ggaggccacgttaagggaatat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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