XB-CLONE-3177120
Sequence:
5' EST: DT396373 [+]
ggaagccaataacactgacttgtacggctcatccagcacctcaagggtgcaagggaaacatcacatggggaggaaggataaacatagagaacatatataa
ctatgagtggaaggatcaggctggtaaagtcacctccatatcagatatcactgttactccatcccagagagagcacaatagccccctaacatgtacagtg
acctatagtaaggtttctactaagaaaaccatcaccctggatattgagtttcctccagaaatgaaaattacagtttcaggatataatggaacttatgaaa
acaaatcagtgactgtgatagagggagacacaaagcttctccgatgtgaagtaaacagcaacccagtggctgaaatttcctgggggagaggagatgagat
tcttagtgaaacatctgggcagggtgtgactcttgcactggagaatgtatcagttgctgatactgtaacctacacgtgcttagcaaggaatactcacggg
tccgtctctgggactgttagtgtcactgtagcatatgccccaagaagaccaactatttcaaactgcacatcaaagggttgccgggttgaagccatagaag
gaacctccctgtccctgctctgctctgctgaaagtaaccctcctgccaacctgtcctgggcaaaaatgggtcagaacagcaccgaccccctaaattcctc
aagtgggagatttgatgtttctcatgtaaccactgataatgngggagattatatgtgccgggcaaggaatanacatgggaattcaacagtatcagtcaca
gttgttatcatttatgctccaagaagactaactattaactactgcacaataaatgattgcttcgagaaaaaaactcttctgtttgagtcttaaagtctgt
tgaagtgttagaaggaaactccctgtacct
3' EST: DT396372 [+]gagatgggtgattaacatttatgccaaagaaaagtaaaaaaaaaaatagaaaagtgcaaaatgccattgctgagcatcctctcattatctgtgttagttc
cgtttgacttctgtatataaagtctcttgtgtttctggagaactttttggttttaactttgagatatcaagggtcacgtattgcagttcttcatcagggc
tttcaccttcatttggagagctgagacccatgctgggttcggctgttcttctgtctatattggcgtaaaccgcttcataattatttggtgatgaacttgt
attttcttcaaccagctctggtggcctcctctttttcttgtatcgcataaccagaactgttacaattactatggcagcgaggagtaggagaaaaattcca
ccaaacattaacccttcacgatggatcttatcatttgtgagagatttgataaccacagtaatagatttactttcatttccaactctgtttgttgcccagc
acatataattccctccattttgagaggtcgcattggtcacattcagttgcgctgagagtaacggttgggtgatgttctgtaaaggttttatccaggacac
atttgccggagggttactgtcagcatcgcactgcaggtacagggagtttccttctaacacttcaacagactttaagacttcaacagaagagtttttttcc
tcgaagcaatcatttattgtgcagtagttaatagttagtcttcttggagcataaatgataacaactgtgactgatactgttgaattcccatgtttattcc
ttgcccggcacatataatctcccccattatcagtggttacatgagaaacatcaaatctcccacttg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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